2. Apoptosis Metabolic Enzyme/Protease
  3. Glutathione Peroxidase
  4. GPX4-IN-2

GPX4-IN-2 is a potent GPX4 inhibitor. GPX4 shows antiproliferative activity. GPX4-IN-2 has the potential for the research of cancer.

For research use only. We do not sell to patients.

GPX4-IN-2 Chemical Structure

GPX4-IN-2 Chemical Structure

CAS No. : 2485005-22-7

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1 mg USD 500 In-stock
5 mg USD 1250 In-stock
10 mg USD 2050 In-stock
25 mg USD 4180 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

GPX4-IN-2 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

GPX4-IN-2 is a potent GPX4 inhibitor. GPX4 shows antiproliferative activity. GPX4-IN-2 has the potential for the research of cancer[1].

IC50 & Target

GPX4[1]
In Vitro

GPX4-IN-2 (compound 28) (0.0005-10 mM; 24 h) shows antiproliferative activity with IC50s of 0.004, 0.016, 2.9 µM for 786-O, SJSA-1, A431 cells, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

GPX4-IN-2 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: SJSA-1, 786-O, A431 cells
Concentration: 0.0005-10 mM
Incubation Time: 24 h
Result: Inhibited cell proliferation with IC50s of 0.004, 0.016, 2.9 µM for 786-O, SJSA-1, A431 cells, respectively.
In Vivo

GPX4-IN-2 (5 mg/kg for mouse, 2 mg/kg for rats; i.v.) shows a good pharmacokinetics profile in mice and rats[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 6-8 weeks, 22-25 g, Male Balb/c mice, 6-8 weeks, 200-250 g, male SD rats[1]
Dosage: 5 mg/kg for mouse, 2 mg/kg for rats
Administration: I.v.
Result: Showed a good pharmacokinetics with T1/2 of 3.5 h, Cmax of 5446 ng/mL, AUC of 1635 ng·h/mL, CL of 49 mL/min/kg, Vd of 14.7 L/kg in mice, T1/2 of 3.15 h, Cmax of 3529 ng/mL, AUC of 1082 ng·h/mL, CL of 30 mL/min/kg, Vd of 8.2 L/kg in rats.
Molecular Weight

444.65

Formula

C30H40N2O
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

CCCC[C@@H]1N[C@H](C(C(C1)=C2)=CC=C2OC)C3=CC=C(C=C3)NC4(C[C@H](C5)C6)C[C@H]6C[C@H]5C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 37.5 mg/mL (84.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2490 mL 11.2448 mL 22.4896 mL
5 mM 0.4498 mL 2.2490 mL 4.4979 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 3.75 mg/mL (8.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2490 mL 11.2448 mL 22.4896 mL 56.2240 mL
5 mM 0.4498 mL 2.2490 mL 4.4979 mL 11.2448 mL
10 mM 0.2249 mL 1.1245 mL 2.2490 mL 5.6224 mL
15 mM 0.1499 mL 0.7497 mL 1.4993 mL 3.7483 mL
20 mM 0.1124 mL 0.5622 mL 1.1245 mL 2.8112 mL
25 mM 0.0900 mL 0.4498 mL 0.8996 mL 2.2490 mL
30 mM 0.0750 mL 0.3748 mL 0.7497 mL 1.8741 mL
40 mM 0.0562 mL 0.2811 mL 0.5622 mL 1.4056 mL
50 mM 0.0450 mL 0.2249 mL 0.4498 mL 1.1245 mL
60 mM 0.0375 mL 0.1874 mL 0.3748 mL 0.9371 mL
80 mM 0.0281 mL 0.1406 mL 0.2811 mL 0.7028 mL
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GPX4-IN-2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GPX4-IN-22485005-22-7Glutathione PeroxidaseSJSA-1786-OA431 cellsantiproliferativeInhibitorinhibitorinhibit

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