GSK-3β-IN-29
GSK-3β-IN-29 is a selective GSK-3β inhibitor with an IC50 of 34.0 nM. GSK-3β-IN-29 shows selectivity for GSK-3β over 40 other kinases and for 5-HT1a receptor. GSK-3β-IN-29 exhibits neuroprotection in mice. GSK-3β-IN-29 can be used for the research of nervous system (CNS) disorders.
For research use only. We do not sell to patients.
- CAS No.: 871843-09-3
- Formula: C21H18N4O
- Molecular Weight:342.39
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
GSK-3β 34.0 nM (IC50) |
In Vitro
GSK-3β-IN-29 (compound 3a) (10-100 μM) shows high selectivity for GSK-3β and GSK-3α over 40 other purified kinases, with selectivity indexes >53 for all non-GSK-3 kinases tested[1].
GSK-3β-IN-29 (50 nM-20 μM; 48 h) provides dose-dependent neuroprotection to neurons against homocysteic acid (HCA)-induced oxidative stress without toxicity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:neurons exposed to homocysteic acid-induced oxidative stress
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Concentration:50; 100; 150; 200; 500 nM; 1; 5; 20 μM
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Incubation Time:48 h
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Result:Showed full neuroprotective activity at concentrations from 1 μM to 20 μM with no observable toxicity.
Achieved nearly complete protection against HCA-induced cell death at 150 nM.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J mice (male, 8 weeks old)[1]
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Dosage:150 mg/kg
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Administration:i.p.; single dose
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Result:Attenuated locomotor activity induced by the Amphetamine + Chlordiazepoxide mixture.
Did not affect locomotor activity induced by amphetamine alone.
Chemical Information
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CAS No. 871843-09-3
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Molecular Weight 342.39
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Formula C21H18N4O
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SMILES
O=C1NNC(C2=CN(C)C3=C2C=CC=C3)=C1C4=CN(C)C5=C4C=CC=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
GSK-3β-IN-29 Related Classifications
HY-183908 Related Classifications
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