2. Metabolic Enzyme/Protease
  3. Aminopeptidase
  4. H-Ala-Pro-Ala-OH

H-Ala-Pro-Ala-OH, tripeptide, is a substrate for Escherichia coli PepP (Xaa-Pro aminopeptidase). H-Ala-Pro-Ala-OH acts as a nitrogen source for wild-type Escherichia coli.

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H-Ala-Pro-Ala-OH

H-Ala-Pro-Ala-OH Chemical Structure

CAS No. : 61430-14-6

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H-Ala-Pro-Ala-OH Related Antibodies

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Description

H-Ala-Pro-Ala-OH, tripeptide, is a substrate for Escherichia coli PepP (Xaa-Pro aminopeptidase). H-Ala-Pro-Ala-OH acts as a nitrogen source for wild-type Escherichia coli[1].

In Vitro

H-Ala-Pro-Ala-OH is cleaved by purified recombinant Escherichia coli PepP with high efficiency, as indicated by a kcat/KM of 1.27 × 104 /s/M[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

H-Ala-Pro-Ala-OH Related Antibodies
Molecular Weight

257.29

Formula

C11H19N3O4
CAS No.
Appearance

Solid

Sequence

Ala-Pro-Ala
Sequence Shortening

APA
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (388.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8867 mL 19.4333 mL 38.8666 mL
5 mM 0.7773 mL 3.8867 mL 7.7733 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8867 mL 19.4333 mL 38.8666 mL 97.1666 mL
5 mM 0.7773 mL 3.8867 mL 7.7733 mL 19.4333 mL
10 mM 0.3887 mL 1.9433 mL 3.8867 mL 9.7167 mL
15 mM 0.2591 mL 1.2956 mL 2.5911 mL 6.4778 mL
20 mM 0.1943 mL 0.9717 mL 1.9433 mL 4.8583 mL
25 mM 0.1555 mL 0.7773 mL 1.5547 mL 3.8867 mL
30 mM 0.1296 mL 0.6478 mL 1.2956 mL 3.2389 mL
40 mM 0.0972 mL 0.4858 mL 0.9717 mL 2.4292 mL
50 mM 0.0777 mL 0.3887 mL 0.7773 mL 1.9433 mL
60 mM 0.0648 mL 0.3239 mL 0.6478 mL 1.6194 mL
80 mM 0.0486 mL 0.2429 mL 0.4858 mL 1.2146 mL
100 mM 0.0389 mL 0.1943 mL 0.3887 mL 0.9717 mL
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H-Ala-Pro-Ala-OH Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

H-Ala-Pro-Ala-OH61430-14-6AminopeptidaseEscherichia coliEscherichia coli PepPXaa-Pro aminopeptidaseInhibitorinhibitorinhibit

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