iMDK quarterhydrate

Name: iMDK quarterhydrate
Brand: MedChemExpress
SKU: HY-110171A
Rating: 5.0 (5 reviews)

Cat. No.: HY-110171A Purity: 99.37%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

iMDK quarterhydrate is a potent PI3K inhibitor and inhibits the growth factor MDK (also known as midkine or MK). iMDK quarterhydrate suppresses non-small cell lung cancer (NSCLC) cooperatively with A MEK inhibitor without harming normal cells and mice.

For research use only. We do not sell to patients.

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of iMDK quarterhydrate:

iMDK In-stock

5 Publications Citing Use of MCE iMDK quarterhydrate

In Vivo Efficacy Study

Histological Imaging/Staining

RT-PCR

Cell Proliferation/Viability Assay

: iMDK quarterhydrate purchased from MedChemExpress. Usage Cited in: Cell Discov. 2024 Oct 22;10(1):110. [Abstract]; iMDK (9 mg/kg, intraperitoneally injected, once a day from E7.5 to E12.5.). Placental efficiency as expressed by the ratio of fetal weight to placental weight (n = 24 for control, n = 41 for iMDK).

: iMDK quarterhydrate purchased from MedChemExpress. Usage Cited in: Cell Discov. 2024 Oct 22;10(1):110. [Abstract]; iMDK (9 mg/kg, intraperitoneally injected, once a day from E7.5 to E12.5.). Measurements of the labyrinth area and JZ area and the ratio of the labyrinth area to the JZ area (n = 8 control, n = 14 iMDK).

: iMDK quarterhydrate purchased from MedChemExpress. Usage Cited in: Cell Discov. 2024 Oct 22;10(1):110. [Abstract]; iMDK (9 mg/kg, intraperitoneally injected, once a day from E7.5 to E12.5.). Representative image of a cross-section through the labyrinth and JZ in a mid-section of a mouse placenta. The blue solid line outlines the labyrinth and JZ that was measured.

: iMDK quarterhydrate purchased from MedChemExpress. Usage Cited in: Cell Discov. 2024 Oct 22;10(1):110. [Abstract]; iMDK (9 mg/kg, intraperitoneally injected, once a day from E7.5 to E12.5.). Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of the expression of markers of SynT-I (Mct1) and endothelial cells (Cd31). RT-qPCR data were normalized to the reference gene Hprt.

: iMDK quarterhydrate purchased from MedChemExpress. Usage Cited in: Cell Discov. 2024 Oct 22;10(1):110. [Abstract]; The CCK-8 assay was used to evaluate the proliferation of endothelial cells treated with iMDK (0.125-1 μM).

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PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

iMDK (50-500 nM) quarterhydrate suppressed AKT phosphorylation in a dose-dependent manner in H441 lung adenocarcinoma cells after treatment for 72 h. In contrast, iMDK quarterhydrate robustly increases p-ERK^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	H441 (lung adenocarcinoma; KRAS^G12V), H2009 (non-small cell carcinoma; KRAS^G12A), A549 (lung carcinoma; KRAS^G12S) and H520 (lung squamous cell carcinoma; KRAS^WT)
Concentration:	iMDK (2.5 μM) quarterhydrate and PD0325901 (0.5 μM) for H441 and H2009 cells iMDK (0.125 μM) quarterhydrate and PD0325901 (0.25 μM) for H520 cells iMDK (0.25 μM) quarterhydrate and PD0325901 (0.125 μM) for A549 cells
Incubation Time:	72 hours
Result:	iMDK quarterhydrate alone did not inhibit cell viability of A549 cells, the combinatorial treatment of iMDK quarterhydrate with PD0325901 significantly inhibited that of A549 cells compared to the single treatment of PD0325901.

Western Blot Analysis^[1]

Cell Line:	H441 lung adenocarcinoma cells
Concentration:	0-500 nM
Incubation Time:	72 hours
Result:	Suppressed AKT phosphorylation in a dose-dependent manner. ERK1/2 phosphorylation was increased.

In Vivo

The combination treatment of iMDK ( (9 mg/kg/day; intraperitoneally injected with 100 μl) and PD0325901 (5 mg/kg; orally administered) effectively reduced lung tumor growth in a xenograft mouse model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	female BALB/c nude mice (6 week old) bearing H441 human lung cancer xenografts^[1]
Dosage:	iMDK (9 mg/kg) quarterhydrate and PD0325901 (5 mg/kg)
Administration:	Intraperitoneally injected with 100 μL iMDK everyday and/or orally
Result:	Reduced significantly volume of the tumors derived from H441 lung adenocarcinoma cells after the combination treatment with iMDK quarterhydrate and PD0325901 compared to that of single compound in a xenograft mouse model.

Molecular Weight

380.91

Formula

C₂₁H₁₃FN₂O₂S.1/4H₂O

Appearance

Solid

Color

White to light yellow

SMILES

O=C1C(C2=CN3C(SC(CC4=CC=C(F)C=C4)=C3)=N2)=CC5=CC=CC=C5O1.O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 3.33 mg/mL (8.74 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6253 mL	13.1265 mL	26.2529 mL
5 mM	0.5251 mL	2.6253 mL	5.2506 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: Corn Oil
Solubility: 2 mg/mL (5.25 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 15% Solutol HS 15 10% Cremophor EL 35% PEG400 40% Water
Solubility: 5 mg/mL (13.13 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.37%

Select Batch:

Data Sheet (278 KB) SDS (252 KB)

COA (250 KB) HNMR (284 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Naomasa Ishida, et al. A novel PI3K inhibitor iMDK suppresses non-small cell lung Cancer cooperatively with A MEK inhibitor. Exp Cell Res. 2015 Jul 15;335(2):197-206. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6253 mL	13.1265 mL	26.2529 mL	65.6323 mL
DMSO	5 mM	0.5251 mL	2.6253 mL	5.2506 mL	13.1265 mL