Inosine-5'-triphosphate trisodium salt
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Inosine-5'-triphosphate trisodium salt is a nucleotide analogue that acts on multiple G proteins and is widely used in G protein-related research. It can bind to the α -subunit of G proteins and participate in G protein-mediated signal transduction as a substitute for GTP. Its mechanism of action is to interact with the nucleotide-binding site of the G protein α -subunit, affecting the activity and function of G proteins. In the research field, it is mainly used to explore the role of the G protein signaling pathway in cellular physiological and pathological processes. For example, in HL-60 leukemia cells, its impact on G protein-mediated signal transduction can be studied.
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- 純度: 99.09%
- CAS 番号: 35908-31-7
- 分子式: C10H12N4Na3O14P3
- 分子量:574.11
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保管条件:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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Endogenous Metabolite アイソフォーム固有の製品をすべて表示
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生物活性
In the ATPase, GTPase, ITPase and XTPase activity assays, the Vmax of inosine-5'-triphosphate trisodium salt hydrolysis by transducin (TD) was lower than that of GTP, and the Km of inosine-5'-triphosphate trisodium salt hydrolysis catalyzed by TD was higher than that of GTP[3].
In the chemokine-stimulated GTPase and ITPase assays on HL-60 cell membranes, fMLP and LTB4 stimulated ITP hydrolysis more strongly than GTP hydrolysis in the presence of inosine-5'-triphosphate trisodium salt[3].
In the bladder assay, 1 mM inosine-5'-triphosphate trisodium salt caused contraction of guinea pig bladder[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
化学情報
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CAS 番号 35908-31-7
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性状 Solid
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分子量 574.11
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分子式 C10H12N4Na3O14P3
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Color White to off-white
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SMILES
O[C@@H]([C@H](N1C=NC2=C1NC=NC2=O)O3)[C@H](O)[C@H]3COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O
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別名
イノシン 5′-三リン酸 三ナトリウム塩
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
H2O : 100 mg/mL (174.18 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (276 KB)
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SDS (393 KB)
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- Deutsch - DE (393 KB)
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- Italian - IT (393 KB)
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- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Hanson RL, et al. Energy-transducing adenosine triphosphatase from Escherichia coli: purification, properties, and inhibition by antibody. J Bacteriol. 1973 May;114(2):772-81. [Content Brief]
[2]. Pieper U, et al. The GTP-binding domain of McrB: more than just a variation on a common theme? J Mol Biol. 1999 Sep 24;292(3):547-56. [Content Brief]
[3]. Klinker JF, et al. Functionally nonequivalent interactions of guanosine 5'-triphosphate, inosine 5'-triphosphate, and xanthosine 5'-triphosphate with the retinal G-protein, transducin, and with Gi-proteins in HL-60 leukemia cell membranes. Biochem Pharmacol. 1997 Sep 1;54(5):551-62. [Content Brief]
[4]. Burnstock G, et al. Atropine resistant excitation of the urinary bladder: the possibility of transmission via nerves releasing a purine nucleotide. Br J Pharmacol. 1972 Mar;44(3):451-61. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.7418 mL | 8.7091 mL | 17.4183 mL | 43.5457 mL |
| 5 mM | 0.3484 mL | 1.7418 mL | 3.4837 mL | 8.7091 mL | |
| 10 mM | 0.1742 mL | 0.8709 mL | 1.7418 mL | 4.3546 mL | |
| 15 mM | 0.1161 mL | 0.5806 mL | 1.1612 mL | 2.9030 mL | |
| 20 mM | 0.0871 mL | 0.4355 mL | 0.8709 mL | 2.1773 mL | |
| 25 mM | 0.0697 mL | 0.3484 mL | 0.6967 mL | 1.7418 mL | |
| 30 mM | 0.0581 mL | 0.2903 mL | 0.5806 mL | 1.4515 mL | |
| 40 mM | 0.0435 mL | 0.2177 mL | 0.4355 mL | 1.0886 mL | |
| 50 mM | 0.0348 mL | 0.1742 mL | 0.3484 mL | 0.8709 mL | |
| 60 mM | 0.0290 mL | 0.1452 mL | 0.2903 mL | 0.7258 mL | |
| 80 mM | 0.0218 mL | 0.1089 mL | 0.2177 mL | 0.5443 mL | |
| 100 mM | 0.0174 mL | 0.0871 mL | 0.1742 mL | 0.4355 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Inosine-5'-triphosphate trisodium salt 関連分類
HY-W013706 関連分類
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