Inosine-5'-triphosphate trisodium salt
Based on 1 Customer Validation
Inosine-5'-triphosphate trisodium salt is a nucleotide analogue that acts on multiple G proteins and is widely used in G protein-related research. It can bind to the α -subunit of G proteins and participate in G protein-mediated signal transduction as a substitute for GTP. Its mechanism of action is to interact with the nucleotide-binding site of the G protein α -subunit, affecting the activity and function of G proteins. In the research field, it is mainly used to explore the role of the G protein signaling pathway in cellular physiological and pathological processes. For example, in HL-60 leukemia cells, its impact on G protein-mediated signal transduction can be studied.
For research use only. We do not sell to patients.
- Purity: 99.09%
- CAS No.: 35908-31-7
- Formula: C10H12N4Na3O14P3
- Molecular Weight:574.11
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-12695
Guanosine 5'-triphosphate trisodium
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HY-D0199
Adenosine 5'-diphosphate disodium
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HY-W011012
Adenosine 5'-monophosphate disodium
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HY-D0199R
Adenosine 5'-diphosphate disodium (Standard)
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HY-108213
Inosinic acid
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HY-A0181
Adenosine monophosphate
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HY-B0345A
ATP disodium salt
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HY-B1511
Cyclic AMP
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HY-W013093
Uridine triphosphate trisodium salt
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HY-W013100
Cytidine-5'-triphosphate disodium
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HY-101981
Uridine 5'-monophosphate
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HY-B1511R
Cyclic AMP (Standard)
All Endogenous Metabolite Isoforms
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Biological Activity
In the ATPase, GTPase, ITPase and XTPase activity assays, the Vmax of inosine-5'-triphosphate trisodium salt hydrolysis by transducin (TD) was lower than that of GTP, and the Km of inosine-5'-triphosphate trisodium salt hydrolysis catalyzed by TD was higher than that of GTP[3].
In the chemokine-stimulated GTPase and ITPase assays on HL-60 cell membranes, fMLP and LTB4 stimulated ITP hydrolysis more strongly than GTP hydrolysis in the presence of inosine-5'-triphosphate trisodium salt[3].
In the bladder assay, 1 mM inosine-5'-triphosphate trisodium salt caused contraction of guinea pig bladder[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 35908-31-7
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Appearance Solid
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Molecular Weight 574.11
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Formula C10H12N4Na3O14P3
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Color White to off-white
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SMILES
O[C@@H]([C@H](N1C=NC2=C1NC=NC2=O)O3)[C@H](O)[C@H]3COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O
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Synonyms
ITP trisodium salt; Inosine triphosphate trisodium salt
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 100 mg/mL (174.18 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Hanson RL, et al. Energy-transducing adenosine triphosphatase from Escherichia coli: purification, properties, and inhibition by antibody. J Bacteriol. 1973 May;114(2):772-81. [Content Brief]
[2]. Pieper U, et al. The GTP-binding domain of McrB: more than just a variation on a common theme? J Mol Biol. 1999 Sep 24;292(3):547-56. [Content Brief]
[3]. Klinker JF, et al. Functionally nonequivalent interactions of guanosine 5'-triphosphate, inosine 5'-triphosphate, and xanthosine 5'-triphosphate with the retinal G-protein, transducin, and with Gi-proteins in HL-60 leukemia cell membranes. Biochem Pharmacol. 1997 Sep 1;54(5):551-62. [Content Brief]
[4]. Burnstock G, et al. Atropine resistant excitation of the urinary bladder: the possibility of transmission via nerves releasing a purine nucleotide. Br J Pharmacol. 1972 Mar;44(3):451-61. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.7418 mL | 8.7091 mL | 17.4183 mL | 43.5457 mL |
| 5 mM | 0.3484 mL | 1.7418 mL | 3.4837 mL | 8.7091 mL | |
| 10 mM | 0.1742 mL | 0.8709 mL | 1.7418 mL | 4.3546 mL | |
| 15 mM | 0.1161 mL | 0.5806 mL | 1.1612 mL | 2.9030 mL | |
| 20 mM | 0.0871 mL | 0.4355 mL | 0.8709 mL | 2.1773 mL | |
| 25 mM | 0.0697 mL | 0.3484 mL | 0.6967 mL | 1.7418 mL | |
| 30 mM | 0.0581 mL | 0.2903 mL | 0.5806 mL | 1.4515 mL | |
| 40 mM | 0.0435 mL | 0.2177 mL | 0.4355 mL | 1.0886 mL | |
| 50 mM | 0.0348 mL | 0.1742 mL | 0.3484 mL | 0.8709 mL | |
| 60 mM | 0.0290 mL | 0.1452 mL | 0.2903 mL | 0.7258 mL | |
| 80 mM | 0.0218 mL | 0.1089 mL | 0.2177 mL | 0.5443 mL | |
| 100 mM | 0.0174 mL | 0.0871 mL | 0.1742 mL | 0.4355 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Inosine-5'-triphosphate trisodium salt Related Classifications
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