2. NF-κB Metabolic Enzyme/Protease MAPK/ERK Pathway Stem Cell/Wnt Immunology/Inflammation
  3. Keap1-Nrf2 Heme Oxygenase (HO) JNK ERK p38 MAPK NF-κB Interleukin Related
  4. Isostrictiniin

Isostrictiniin (ITN) is a polyphenolic compound isolated from Nymphaea candida. It upregulates the expression of Nrf2 and HO-1, while downregulating the expression of Keap1. Isostrictiniin reduces the phosphorylation levels of JNK, ERK1/2, p38, IκBα, and NF-κB p65. Isostrictiniin decreases the levels of pro-inflammatory mediators (IL-1β, IL-6, TNF-α, PGE2). Isostrictiniin alleviates LPS-induced acute lung injury and exerts protective effects against acute alcoholic liver injury. Additionally, isostrictiniin exhibits anti-inflammatory, antioxidant, and anti-fibrotic activities.

For research use only. We do not sell to patients.

Isostrictiniin

Isostrictiniin Chemical Structure

CAS No. : 1801746-41-7

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Isostrictiniin Related Antibodies

Top Publications Citing Use of Products

View All Heme Oxygenase (HO) Isoform Specific Products:

View All Isoforms
HO-1 HO-2

View All JNK Isoform Specific Products:

View All Isoforms
JNK1 JNK2 JNK3 JNK

View All ERK Isoform Specific Products:

View All Isoforms
ERK ERK1 ERK2 ERK5 ERK8

View All p38 MAPK Isoform Specific Products:

View All Isoforms
p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All Interleukin Related Isoform Specific Products:

View All Isoforms
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Isostrictiniin (ITN) is a polyphenolic compound isolated from Nymphaea candida. It upregulates the expression of Nrf2 and HO-1, while downregulating the expression of Keap1. Isostrictiniin reduces the phosphorylation levels of JNK, ERK1/2, p38, IκBα, and NF-κB p65. Isostrictiniin decreases the levels of pro-inflammatory mediators (IL-1β, IL-6, TNF-α, PGE2). Isostrictiniin alleviates LPS-induced acute lung injury and exerts protective effects against acute alcoholic liver injury. Additionally, isostrictiniin exhibits anti-inflammatory, antioxidant, and anti-fibrotic activities.

IC50 & Target[2]

ERK1

 

ERK2

 

p38

 

IL-6

 

IL-1β

 

In Vitro

Isostrictiniin (50-200 μM; 12 h) impairs proliferation of A549 cells, reducing cell survival rate below 80%[2].
Isostrictiniin (25 μM; 12 h) reduces pro-inflammatory mediator levels (IL-1β, IL-6, TNF-α, PGE2) in LPS-induced A549 cells[2].
Isostrictiniin (10-25 μM; 12 h) upregulates Nrf2 and HO-1, and reduces Keap1 in LPS-induced A549 cells, regulating the Keap1-Nrf2/HO-1 pathway[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Isostrictiniin Related Antibodies

Western Blot Analysis[2]

Cell Line: LPS-induced A549
Concentration: 10-25 μM (with LPS)
Incubation Time: 12 h
Result: Upregulated Nrf2 and HO-1 protein expressions (p < 0.05) at both concentrations; markedly reduced Keap1 protein expression (p < 0.05) at 25 μM compared to the model group.

Western Blot Analysis[2]

Cell Line: LPS-induced A549
Concentration: 5-25 μM (with LPS)
Incubation Time: 12 h
Result: Reduced phosphorylated protein expressions of JNK, ERK1/2, p38, IκBα, and NF-κB p65 (p < 0.05) compared to the model group.
In Vivo

Isostrictiniin (25-100 mg/kg; intragastric; once daily; 7 days) alleviates LPS-induced acute lung injury in mice by reducing inflammatory responses, oxidative stress, and histopathological damage in a dose-dependent manner[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Kunming (weight 18–20 g)[2]
Dosage: 25, 50, 100 mg/kg
Administration: intragastric; once daily; 7 days
Result: Significantly reduced lung indexes (p < 0.05), lung wet-to-dry (W/D) ratio, bronchoalveolar lavage fluid (BALF) protein leakage, and BALF white blood cell (WBC) counts (p < 0.01) at 25, 50, 100 mg/kg. Reduced lung tissue myeloperoxidase (MPO) activity (p < 0.05), lung tissue tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) levels (p < 0.01) at 50, 100 mg/kg. Suppressed blood neutrophil counts, elevated lymphocyte counts (p < 0.01), reduced lung tissue malondialdehyde (MDA) levels, and increased superoxide dismutase (SOD) and glutathione (GSH) levels (p < 0.05) at 100 mg/kg. Improved lung histopathological damage and reduced pathological scores (p < 0.05) at 25, 50, 100 mg/kg.
Molecular Weight

634.45

Formula

C27H22O18
CAS No.
SMILES

OC1=C2C3=C(O)C(O)=C(O)C=C3C(O[C@]4([H])[C@@]([C@@H]([C@H](O[C@H]4OC(C5=CC(O)=C(C(O)=C5)O)=O)CO)O)([H])OC(C2=CC(O)=C1O)=O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Isostrictiniin Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isostrictiniin1801746-41-7ITNKeap1-Nrf2Heme Oxygenase (HO)JNKERKp38 MAPKNF-κBInterleukin RelatedHeme Oxygenasec-Jun N-terminal kinaseExtracellular signal regulated kinasesNuclear factor-κBNuclear factor-kappaBILNF-κB p65A549 cellsmouse modelsHO-1Nrf2p38IκBαERK1/2Keap1Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Isostrictiniin
Cat. No.:
HY-N17317
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.