Isostrictiniin
Isostrictiniin (ITN) is a polyphenolic compound isolated from Nymphaea candida. It upregulates the expression of Nrf2 and HO-1, while downregulating the expression of Keap1. Isostrictiniin reduces the phosphorylation levels of JNK, ERK1/2, p38, IκBα, and NF-κB p65. Isostrictiniin decreases the levels of pro-inflammatory mediators (IL-1β, IL-6, TNF-α, PGE2). Isostrictiniin alleviates LPS-induced acute lung injury and exerts protective effects against acute alcoholic liver injury. Additionally, isostrictiniin exhibits anti-inflammatory, antioxidant, and anti-fibrotic activities.
For research use only. We do not sell to patients.
- CAS No.: 1801746-41-7
- Formula: C27H22O18
- Molecular Weight:634.45
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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ERK1 |
ERK2 |
p38 |
IL-6 |
IL-1β |
Isostrictiniin (50-200 μM; 12 h) impairs proliferation of A549 cells, reducing cell survival rate below 80%[2].
Isostrictiniin (25 μM; 12 h) reduces pro-inflammatory mediator levels (IL-1β, IL-6, TNF-α, PGE2) in LPS-induced A549 cells[2].
Isostrictiniin (10-25 μM; 12 h) upregulates Nrf2 and HO-1, and reduces Keap1 in LPS-induced A549 cells, regulating the Keap1-Nrf2/HO-1 pathway[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LPS-induced A549
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Concentration:10-25 μM (with LPS)
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Incubation Time:12 h
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Result:Upregulated Nrf2 and HO-1 protein expressions (p < 0.05) at both concentrations; markedly reduced Keap1 protein expression (p < 0.05) at 25 μM compared to the model group.
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Cell Line:LPS-induced A549
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Concentration:5-25 μM (with LPS)
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Incubation Time:12 h
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Result:Reduced phosphorylated protein expressions of JNK, ERK1/2, p38, IκBα, and NF-κB p65 (p < 0.05) compared to the model group.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Kunming (weight 18–20 g)[2]
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Dosage:25, 50, 100 mg/kg
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Administration:intragastric; once daily; 7 days
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Result:Significantly reduced lung indexes (p < 0.05), lung wet-to-dry (W/D) ratio, bronchoalveolar lavage fluid (BALF) protein leakage, and BALF white blood cell (WBC) counts (p < 0.01) at 25, 50, 100 mg/kg. Reduced lung tissue myeloperoxidase (MPO) activity (p < 0.05), lung tissue tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) levels (p < 0.01) at 50, 100 mg/kg. Suppressed blood neutrophil counts, elevated lymphocyte counts (p < 0.01), reduced lung tissue malondialdehyde (MDA) levels, and increased superoxide dismutase (SOD) and glutathione (GSH) levels (p < 0.05) at 100 mg/kg. Improved lung histopathological damage and reduced pathological scores (p < 0.05) at 25, 50, 100 mg/kg.
Chemical Information
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CAS No. 1801746-41-7
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Molecular Weight 634.45
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Formula C27H22O18
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SMILES
OC1=C2C3=C(O)C(O)=C(O)C=C3C(O[C@]4([H])[C@@]([C@@H]([C@H](O[C@H]4OC(C5=CC(O)=C(C(O)=C5)O)=O)CO)O)([H])OC(C2=CC(O)=C1O)=O)=O
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Synonyms
ITN
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Guo Z, et al. Protective effect of isostrictiniin on alcoholic liver injury in mice[J]. 2023.
[2]. Ding W, et al. Isostrictiniin Alleviates LPS-Induced Acute Lung Injury via the Regulation of the Keap1-Nrf2/HO-1 and MAPK/NF-κB Signaling Pathways. Int J Mol Sci. 2025;26(12):5912. Published 2025 Jun 19. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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