Isotianil
Based on 1 Customer Validation
Isotianil is a plant defense inducer that has no direct antimicrobial effect but instead activates typical plant defense responses. Isotianil can be used as a plant protection agent for controlling rice blast.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.67%
- CAS. Nr.: 224049-04-1
- Formel: C11H5Cl2N3OS
- Molecular Weight:298.15
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Speicherung:
RT, protect from light.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
Isotianil (0.1-100 ppm, 2 days) can induce the accumulation of endogenous plant resistance-related enzymes such as lipoxygenase, phenylalanine ammonia lyase and chitinase in rice tissues. Induction of disease resistance-related genes, including WRKY45[1].
Isotianil has a good preventive effect on leaf blast at a lower dose, and has shown plant resistance induction against various bacterial diseases, including bacterial seedling rot caused by Burkholderia[1].
Isotianil effectively protects seedling wheat plants against the virulent MoT isolate BR32, preventing the invasive growth of MoT from progressing from the initially attacked epidermal cells to the mesophyll[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS. Nr. 224049-04-1
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Appearance Solid
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Molecular Weight 298.15
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Formel C11H5Cl2N3OS
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Color Off-white to light yellow
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SMILES
O=C(C1=C(Cl)C(Cl)=NS1)NC2=CC=CC=C2C#N
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
RT, protect from light
In solvent -80°C 1 year -20°C 6 months
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 87.5 mg/mL (293.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (16.77 mM); Suspended solution
This protocol yields a suspended solution of ≥ 5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
Reinheit & Dokumentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.3540 mL | 16.7701 mL | 33.5402 mL | 83.8504 mL |
| 5 mM | 0.6708 mL | 3.3540 mL | 6.7080 mL | 16.7701 mL | |
| 10 mM | 0.3354 mL | 1.6770 mL | 3.3540 mL | 8.3850 mL | |
| 15 mM | 0.2236 mL | 1.1180 mL | 2.2360 mL | 5.5900 mL | |
| 20 mM | 0.1677 mL | 0.8385 mL | 1.6770 mL | 4.1925 mL | |
| 25 mM | 0.1342 mL | 0.6708 mL | 1.3416 mL | 3.3540 mL | |
| 30 mM | 0.1118 mL | 0.5590 mL | 1.1180 mL | 2.7950 mL | |
| 40 mM | 0.0839 mL | 0.4193 mL | 0.8385 mL | 2.0963 mL | |
| 50 mM | 0.0671 mL | 0.3354 mL | 0.6708 mL | 1.6770 mL | |
| 60 mM | 0.0559 mL | 0.2795 mL | 0.5590 mL | 1.3975 mL | |
| 80 mM | 0.0419 mL | 0.2096 mL | 0.4193 mL | 1.0481 mL | |
| 100 mM | 0.0335 mL | 0.1677 mL | 0.3354 mL | 0.8385 mL |
Isotianil Related Classifications
HY-W075256 Related Classifications
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