Peroxyfluor 1
Based on 1 Customer Validation
Peroxyfluor 1 is a cell-permeable probe for H2O2. Peroxyfluor 1 represents a first-generation, green-fluorescent probe.
For research use only. We do not sell to patients.
- Purity: 97.0%
- CAS No.: 817170-66-4
- Formula: C32H34B2O7
- Molecular Weight:552.23
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation[1]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 5 mM (optimized according to the experiment).
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 5 µM with PBS or cell culture medium.
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 Peroxyfluor 1 (5 μM) is incubated with live cell samples for 5-10 min.
2.2 Addition of H2O2 (10-100 μM) to cell samples for 15-30 min.
2.3 Fluorescence microscopy detection (Ex/Em = 450/530 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
530
450
Chemical Information
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CAS No. 817170-66-4
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Appearance Solid
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Molecular Weight 552.23
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Formula C32H34B2O7
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Color White to off-white
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SMILES
O=C1OC2(C3=C1C=CC=C3)C4=CC=C(B5OC(C)(C(C)(C)O5)C)C=C4OC6=CC(B7OC(C)(C(C)(C)O7)C)=CC=C62
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 14.29 mg/mL (25.88 mM; ultrasonic and warming and adjust pH to 10 with 1M NaOH; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8108 mL | 9.0542 mL | 18.1084 mL | 45.2710 mL |
| 5 mM | 0.3622 mL | 1.8108 mL | 3.6217 mL | 9.0542 mL | |
| 10 mM | 0.1811 mL | 0.9054 mL | 1.8108 mL | 4.5271 mL | |
| 15 mM | 0.1207 mL | 0.6036 mL | 1.2072 mL | 3.0181 mL | |
| 20 mM | 0.0905 mL | 0.4527 mL | 0.9054 mL | 2.2635 mL | |
| 25 mM | 0.0724 mL | 0.3622 mL | 0.7243 mL | 1.8108 mL |
Peroxyfluor 1 Related Classifications
HY-D1458 Related Classifications
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