5-Dehydroepisterol
Based on 1 Customer Validation
5-Dehydroepisterol is a C28 alkylated sterol based on ergostane, and serves as a major component of the sterol pool in Leishmania parasites. The content of 5-Dehydroepisterol in Leishmania promastigotes can be depleted by 22,26-Azasterol, Amiodarone (HY-14187) and Tomatine (HY-N2166). Among these agents, Amiodarone (HY-14187) inhibits the biosynthetic process of this sterol by disrupting the function of squalene epoxidase. 5-Dehydroepisterol can be used in the research of leishmaniasis and cutaneous leishmaniasis.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 91.96%
- CAS 番号: 23582-83-4
- 分子式: C28H44O
- 分子量:396.65
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保管条件:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
IC50 & Target
IC50: Intermediate in steroid biosynthesis[1]
体外実験
5-Dehydroepisterol represents 52.3 mass % of free sterols in untreated Leishmania amazonensis promastigotes[1].
5-Dehydroepisterol constitutes 62.5% of total free sterols in untreated Leishmania amazonensis promastigotes[5].
5-Dehydroepisterol is a major endogenous ergostane-based sterol in WT Leishmania major LV39 clone 5 promastigotes, whose production is entirely dependent on functional sterol methyltransferase, with SMT80 being far more effective than SMT90 at restoring its biosynthesis in SMT-null mutants[6].
5-Dehydroepisterol (24 h) is the dominant sterol produced by Leishmania tarentolae promastigotes, and its synthesis is concentration-dependently inhibited by sterol endoperoxides, miconazole, and antimycin A[2].
5-dehydroepisterol constitutes 67% of the neutral lipid fraction in untreated Leishmania mexicana promastigotes[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 23582-83-4
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性状 Solid
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分子量 396.65
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分子式 C28H44O
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Color White to yellow
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SMILES
C=C(C(C)C)CC[C@@H](C)[C@H]1CC[C@@]2([H])C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
溶剤 & 溶解度
体外:
DMSO : 1 mg/mL (2.52 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Rodrigues JC, et al. Ultrastructural and biochemical alterations induced by 22,26-azasterol, a delta(24(25))-sterol methyltransferase inhibitor, on promastigote and amastigote forms of Leishmania amazonensis. Antimicrobial agents and chemotherapy. 2002 Feb;46(2):487-99. [Content Brief]
[2]. Sarkar D, et al. Sterol Endoperoxides and Their Antileishmanial Effects: Influence on Viability, Oxygen Metabolism and Sterol Synthesis. Molecules (Basel, Switzerland). 2026 Mar 14;31(6):979. [Content Brief]
[3]. Serrano-Martín X, et al. Amiodarone destabilizes intracellular Ca2+ homeostasis and biosynthesis of sterols in Leishmania mexicana. Antimicrobial agents and chemotherapy. 2009 Apr;53(4):1403-10. [Content Brief]
[4]. Berman JD, et al. Effects of ketoconazole on sterol biosynthesis by Leishmania mexicana mexicana amastigotes in murine macrophage tumor cells. Molecular and biochemical parasitology. 1986 Jul;20(1):85-92. [Content Brief]
[5]. Medina JM, et al. Tomatidine promotes the inhibition of 24-alkylated sterol biosynthesis and mitochondrial dysfunction in Leishmania amazonensis promastigotes. Parasitology. 2012 Sep;139(10):1253-65. [Content Brief]
[6]. Mukherjee S, et al. Sterol methyltransferase is required for optimal mitochondrial function and virulence in Leishmania major. Molecular microbiology. 2019 Jan;111(1):65-81. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5211 mL | 12.6056 mL | 25.2111 mL | 63.0279 mL |
5-Dehydroepisterol 関連分類
HY-130703 関連分類
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