BODIPY-Cyclopamine
Based on 1 Customer Validation
BODIPY-Cyclopamine is a fluorescently labeled ligand for the Smoothened (SMO) receptor. The activation of SMO is regulated by Patch protein, and over-activated SMO signaling pathways are associated with tumorigenesis. The NanoBRET (Nanofluorescein bioluminescence resonance energy transfer) technique used in the study can sensitively detect the resonance energy transfer between SMO and BODIPY-Cyclopamine, which can be used for high-throughput screening and kinetic analysis. Studying the binding site of BODIPY-Cyclopamine on SMO can also further explore SMO-targeted drugs.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.91%
- CAS 番号: 334658-24-1
- 分子式: C49H70BF2N5O4
- 分子量:841.92
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
化学情報
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CAS 番号 334658-24-1
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性状 Solid
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分子量 841.92
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分子式 C49H70BF2N5O4
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Color Orange to red
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SMILES
C[C@H]1[C@@]2(O[C@@](C[C@@H]3C)([H])[C@@]1([H])N(C3)CCNC(CCCCCNC(CCC4=CC=C(C=C(C(C)=C5)[N]6=C5C)[N-]4[B+3]6([F-])[F-])=O)=O)C(C)=C(C[C@@]7([H])[C@@]8([H])CC=C(C9)[C@@]7(CC[C@@H]9O)C)[C@@]8([H])CC2
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
DMSO : 100 mg/mL (118.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.97 mM); Suspended solution
This protocol yields a suspended solution of ≥ 2.5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
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- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1878 mL | 5.9388 mL | 11.8776 mL | 29.6940 mL |
| 5 mM | 0.2376 mL | 1.1878 mL | 2.3755 mL | 5.9388 mL | |
| 10 mM | 0.1188 mL | 0.5939 mL | 1.1878 mL | 2.9694 mL | |
| 15 mM | 0.0792 mL | 0.3959 mL | 0.7918 mL | 1.9796 mL | |
| 20 mM | 0.0594 mL | 0.2969 mL | 0.5939 mL | 1.4847 mL | |
| 25 mM | 0.0475 mL | 0.2376 mL | 0.4751 mL | 1.1878 mL | |
| 30 mM | 0.0396 mL | 0.1980 mL | 0.3959 mL | 0.9898 mL | |
| 40 mM | 0.0297 mL | 0.1485 mL | 0.2969 mL | 0.7424 mL | |
| 50 mM | 0.0238 mL | 0.1188 mL | 0.2376 mL | 0.5939 mL | |
| 60 mM | 0.0198 mL | 0.0990 mL | 0.1980 mL | 0.4949 mL | |
| 80 mM | 0.0148 mL | 0.0742 mL | 0.1485 mL | 0.3712 mL | |
| 100 mM | 0.0119 mL | 0.0594 mL | 0.1188 mL | 0.2969 mL |
BODIPY-Cyclopamine 関連分類
HY-137115 関連分類
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