DiOC7(3)
DiOC7(3) (3,3'-Diheptyloxacarbocyanine iodide) is a green membrane potential probe (Ex=450-490 nm, Em=510-520 nm). DiOC7(3) can be used to quantify the vascular densities.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 53213-83-5
- 分子式: C31H41IN2O2
- 分子量:600.57
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体内実験
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. The dye is administered to murine via lateral tail vein injection at a dose of 1 mg/kg/ in 50 μL volume.
2. Animals are killed 5 min after dye administration, and tumors are immediately excised and frozen at - 20°C.
3. Frozen sections 10 μm in thickness are rewarmed to room temperature, allowed to air dry.
4. Image using a microscope with 100W mercury lamp illumination and an epifluorescence condenser (excited at 430-490 nm, emitted at 510-520 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
510
Excitation (Ex)
475
化学情報
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CAS 番号 53213-83-5
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分子量 600.57
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分子式 C31H41IN2O2
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SMILES
CCCCCCCN1/C(OC2=CC=CC=C12)=C/C=C/C3=[N+](CCCCCCC)C4=CC=CC=C4O3.[I-]
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別名
3,3'-Diheptyloxacarbocyanine iodide
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Fluorescent plasma-membrane potential dye assay
Fluorescent plasma-membrane potential dye assays measure changes in cell membrane potential using voltage-sensitive dyes whose fluorescence changes when cells depolarize or hyperpolarize. Anionic bis-oxonol dyes such as DiBAC4(3) enter depolarized cells more readily and show increased fluorescence after intracellular binding, while hyperpolarization reduces dye accumulation and fluorescence. FMP/FLIPR membrane-potential dyes are used for faster, homogeneous microplate assays of ion-channel or receptor-mediated membrane-potential changes.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
DiOC7(3) 関連分類
HY-D1073 関連分類
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