Fluchloralin
Fluchloralin is a dinitroaniline herbicide that effectively controls annual gramineous and broadleaf weeds primarily by inhibiting tubulin synthesis and cell division. Fluchloralin exhibits cytotoxicity and genotoxicity, and promotes cell apoptosis by activating apoptotic signaling proteins, forming DNA ladder bands, inducing cell shrinkage and nuclear fragmentation.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 33245-39-5
- 分子式: C12H13ClF3N3O4
- 分子量:355.70
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HFF | IC50 |
0.33 μM
Compound: 6
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Antimicrobial activity against Toxoplasma gondii RH infected in HFF cells by plaque assay
Antimicrobial activity against Toxoplasma gondii RH infected in HFF cells by plaque assay
|
[PMID: 20145086] |
| HFF | IC50 |
0.77 μM
Compound: 6
|
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235V mutation infected in HFF cells by plaque assay
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235V mutation infected in HFF cells by plaque assay
|
[PMID: 20145086] |
| HFF | IC50 |
1 μM
Compound: 6
|
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235L mutation infected in HFF cells by plaque assay
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235L mutation infected in HFF cells by plaque assay
|
[PMID: 20145086] |
| HFF | IC50 |
1.6 μM
Compound: 6
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Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin H28Q mutation infected in HFF cells by plaque assay
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin H28Q mutation infected in HFF cells by plaque assay
|
[PMID: 20145086] |
| HFF | IC50 |
1.9 μM
Compound: 6
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Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin S6I mutation infected in HFF cells by plaque assay
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin S6I mutation infected in HFF cells by plaque assay
|
[PMID: 20145086] |
| HFF | IC50 |
17.7 μM
Compound: 6
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Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin R243S mutation infected in HFF cells by plaque assay
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin R243S mutation infected in HFF cells by plaque assay
|
[PMID: 20145086] |
| HFF | IC50 |
2.1 μM
Compound: 6
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Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235T mutation infected in HFF cells by plaque assay
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235T mutation infected in HFF cells by plaque assay
|
[PMID: 20145086] |
| HFF | IC50 |
2.2 μM
Compound: 6
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Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin R243C mutation infected in HFF cells by plaque assay
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin R243C mutation infected in HFF cells by plaque assay
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[PMID: 20145086] |
| HFF | IC50 |
20.5 μM
Compound: 6
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Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin T239I mutation infected in HFF cells by plaque assay
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin T239I mutation infected in HFF cells by plaque assay
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[PMID: 20145086] |
| HFF | IC50 |
22.2 μM
Compound: 6
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Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin L136F mutation infected in HFF cells by plaque assay
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin L136F mutation infected in HFF cells by plaque assay
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[PMID: 20145086] |
| HFF | IC50 |
3.2 μM
Compound: 6
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Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin F24H mutation infected in HFF cells by plaque assay
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin F24H mutation infected in HFF cells by plaque assay
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[PMID: 20145086] |
| HFF | IC50 |
8 μM
Compound: 6
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Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin V4L mutation infected in HFF cells by plaque assay
Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin V4L mutation infected in HFF cells by plaque assay
|
[PMID: 20145086] |
Fluchloralin (5-20 μg/mL; 24-72 h) exhibits dose- and time-dependent cytotoxicity in CHO and MEF cells[2].
Fluchloralin (2.5-20.0 μg/mL; 4-12 h) induces statistically significant, time-dependent chromosomal aberrations in CHO cells[2].
Fluchloralin (1.0-20.0 μg/mL; 12 h) causes a statistically significant increase in the level of sister chromatid exchange in CHO cells[2].
Fluchloralin (5-20 μg/mL; 30 h) exerts a dose-dependent inhibitory effect on DNA synthesis in synchronized MEF cells without affecting thymidine transport[2].
Fluchloralin (5-20 μg/mL; 6 h) reduces the DNA synthesis level of synchronized MEF cells by nearly 50% at 12 h post-treatment, without affecting thymidine transport[2].
Fluchloralin (5-20 μg/mL; 24-72 h) induces apoptosis in CHO cells, which is evidenced by the formation of DNA ladder bands and typical nuclear changes[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Chinese hamster ovary (CHO) cells, mouse embryo fibroblast (MEF) cells
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Concentration:5, 10, 15 and 20 μg/mL
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Incubation Time:24 h; 48 h; 72 h
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Result:Induced a dose-dependent decrease in cell viability in both cell lines, with greater toxicity observed in CHO cells than MEF cells.
Reduced viability to 50% in CHO cells treated with 20 μg/mL for 72 h.
Reduced viability to 50% in MEF cells treated with 20 μg/mL for 72 h.
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Cell Line:Chinese hamster ovary (CHO) cells
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Concentration:5, 10 and 20 μg/mL
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Incubation Time:24 h; 48 h; 72 h
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Result:Induced a characteristic DNA ladder pattern in agarose gels of DNA from treated CHO cells.
Revealed apoptotic features including cell shrinkage and nuclear fragmentation via microscopic observation.
Induced apoptosis at 20 μg/mL after 72 h of treatment.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Transgenic zebrafish (olig2:dsRed, cmlc2:dsRed, lfabp:dsRed;elastase:GFP); wild-type zebrafish (reared at 26-28.5 °C on a 13 h light/11 h dark cycle, 8 hours post-fertilization)[1]
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Dosage:0.5 mg/L; 1 mg/L; 2 mg/L
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Administration:aquatic immersion; daily; 96 hours
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Result:Significantly increased the number of apoptotic cells in the brain region of zebrafish.
Caused developmental toxicity to multiple organs (nervous system, heart, liver, pancreas) in zebrafish embryos.
化学情報
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CAS 番号 33245-39-5
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分子量 355.70
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分子式 C12H13ClF3N3O4
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SMILES
ClCCN(CCC)C1=C(C=C(C(F)(F)F)C=C1[N+]([O-])=O)[N+]([O-])=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Hong T, et al. Fluchloralin induces developmental toxicity in heart, liver, and nervous system during early zebrafish embryogenesis. Comp Biochem Physiol C Toxicol Pharmacol. 2023;271:109679. [Content Brief]
[2]. Sinha S, et al. Fluchloralin is cytotoxic and genotoxic and induces apoptosis in mammalian cells. Environ Mol Mutagen. 1998;31(3):257-262. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Fluchloralin 関連分類
HY-W714212 関連分類
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