Ganoderic acid AM1
Ganoderic acid AM1 (GAAM1) is a natural product that can be found in ganoderma lucidum. Ganoderic acid AM1 shows cytotoxicity with an IC50 value of 19.8 µM for HeLa cells.
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- CAS 番号: 149507-55-1
- 分子式: C30H42O7
- 分子量:514.65
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Bel-7402 | IC50 |
7.25 μM
Compound: 17
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Cytotoxicity against human Bel7402 cells
Cytotoxicity against human Bel7402 cells
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[PMID: 23092389] |
| HeLa | IC50 |
202 μM
Compound: 17
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Cytotoxicity against human HeLa cells
Cytotoxicity against human HeLa cells
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[PMID: 23092389] |
| P388 | IC50 |
7.25 μM
Compound: 17
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Cytotoxicity against mouse P388 cells
Cytotoxicity against mouse P388 cells
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[PMID: 23092389] |
| SGC-7901 | IC50 |
7.25 μM
Compound: 17
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Cytotoxicity against human SGC7901 cells
Cytotoxicity against human SGC7901 cells
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[PMID: 23092389] |
化学情報
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CAS 番号 149507-55-1
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分子量 514.65
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分子式 C30H42O7
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SMILES
CC(C)([C@](C1)2[H])[C@@H](O)CC[C@]2(C)C3=C([C@@]4(C(C[C@@H]([C@]4(CC3=O)C)[C@H](C)CC(CC(C)C(O)=O)=O)=O)C)C1=O
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別名
GAAM1
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Structure Classification
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Initial Source
Ganoderma lucidum (Curtis) P. Karst.
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Ganoderic acid AM1 関連分類
HY-129148 関連分類
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