MAP3K14-IN-1
MAP3K14-IN-1 (Compound 173) is a MAP3K14 (NIK kinase) inhibitor with an IC50 of 1.8 nM. MAP3K14-IN-1 inhibits the autophosphorylation of MAP3K14 kinase. MAP3K14-IN-1 reduces the level of phosphorylated IKKα in cancer cells. MAP3K14-IN-1 inhibits the proliferation of multiple myeloma cells. MAP3K14-IN-1 can be used in research related to multiple myeloma.
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- CAS 番号: 2113617-02-8
- 分子式: C29H31N7O2
- 分子量:509.61
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
製品説明
IC50 & Target
[1]|
NIK/MAP3K14 1.8 nM (IC50) |
IKKα 1.3 nM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| JJN-3 | IC50 |
29 nM
Compound: 173
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Antiproliferative activity against human JJN-3 cells after 96 hrs by CellTiter-Glo luminescence assay
Antiproliferative activity against human JJN-3 cells after 96 hrs by CellTiter-Glo luminescence assay
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[PMID: 28947934] |
| KMS-12-BM | IC50 |
>10 μM
Compound: 173
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Antiproliferative activity against human KMS-12-BM cells after 96 hrs by CellTiter-Glo luminescence assay
Antiproliferative activity against human KMS-12-BM cells after 96 hrs by CellTiter-Glo luminescence assay
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[PMID: 28947934] |
体外実験
MAP3K14-IN-1 inhibits autophosphorylation of recombinant MAP3K14/NIK kinase in cell-free assays, with an IC50 of 1.8 nM[1].
MAP3K14-IN-1 inhibits the level of p-IKKα in L363 multiple myeloma cells, with an IC50 of 1.3 nM[1].
MAP3K14-IN-1 inhibits the proliferation of JJN-3 multiple myeloma cells with an IC50 of 29 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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CAS 番号 2113617-02-8
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分子量 509.61
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分子式 C29H31N7O2
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SMILES
C(O)[C@]1(C)C=2C(=C(C#N)C=C(C2)C3=NC(NC4=CC(C#N)=C(CCN5CCOCC5)C=C4C)=NC=C3)NC1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
MAP3K14-IN-1 関連分類
HY-124865 関連分類
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