Kaempferol-7-O-β-D-galactopyranoside
Kaempferol-7-O-β-D-galactopyranoside is a flavonoid glycoside found in the aerial parts of Gynostemma pentaphyllum with no significant antiproliferative activity against cancer cells.
For research use only. We do not sell to patients.
- CAS No.: 53585-54-9
- Formula: C21H20O11
- Molecular Weight:448.38
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Kaempferol-7-O-β-D-galactopyranoside (Compound 22) (100 μg/mL; 48 h) shows weak antiproliferative activity against A549 and HepG2 cells, with no measurable EC50 below 100 μg/mL for either cell line[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:human lung adenocarcinoma (A549) cells, human hepatocellular carcinoma (HepG2) cells
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Concentration:100 μg/mL
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Incubation Time:48 h (antiproliferation assessment); 6 h (alamar blue absorbance measurement)
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Result:Inhibited A549 cell proliferation by 22.9%.
Inhibited HepG2 cell proliferation by 26.6%.
Showed no measurable EC50 below 100 μg/mL for either cell line.
Chemical Information
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CAS No. 53585-54-9
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Molecular Weight 448.38
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Formula C21H20O11
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SMILES
O=C1C2=C(O)C=C(O[C@@H]3O[C@@H]([C@@H]([C@@H]([C@H]3O)O)O)CO)C=C2OC(C4=CC=C(C=C4)O)=C1O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Kaempferol-7-O-β-D-galactopyranoside Related Classifications
HY-N9906 Related Classifications
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