Peimisine
Based on 3 publication(s) in Google Scholar
Peimisine (Ebeiensine) is a muscarinic M receptor antagonist and angiotensin converting enzyme (ACE) inhibitor. Peimisine shows anti-tumor, anti-inflammatory, antihypertensive activities. Peimisine can induce apoptosis and be used in cough and asthma research.
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- Purity : 99.51%
- CAS No.: 19773-24-1
- 화학식: C27H41NO3
- 분자량:427.62
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Peimisine
More
Customer Validation & Images
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In Vivo Efficacy Study
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Histological Imaging/Staining
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WB
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IF
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ELISA
Biological Activity
제품 설명
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| A549 | CC50 |
9.6 μM
Compound: 20
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Cytotoxicity against human A549 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 method
Cytotoxicity against human A549 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 method
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[PMID: 38294199] |
| NCI-H460 | CC50 |
17 μM
Compound: 20
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Cytotoxicity against human NCI-H460 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 method
Cytotoxicity against human NCI-H460 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 method
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[PMID: 38294199] |
In Vitro
Peimisine (17.43-92.07 μg/mL; 72 h) shows significant cytotoxic effects[3].
Peimisine (15 μg/mL; 24, 48 and 72 h) induces G0/G1 phase arrest and rising apoptosis rate[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:A2780 cells
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Concentration:15 μg/mL
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Incubation Time:24, 48 and 72 hours
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Result:Induced G0/G1 phase arrest of A2780 cells in a time-dependent manner.
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Cell Line:LLC, A2780, HepG2 and A549 cells
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Concentration:17.43-92.07 μg/mL
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Incubation Time:72 hours
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Result:Inhibited LLC, A2780, HepG2 and A549 cells with the IC50 values of 20.75 μg/mL, 17.43 μg/mL, 92.07 μg/mL, 36.11 μg/mL, respectively.
Chemical Information
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CAS No. 19773-24-1
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Appearance Solid
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분자량 427.62
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화학식 C27H41NO3
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Color White to off-white
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SMILES
O=C1[C@](C[C@@H](O)CC2)([H])[C@@]2(C)[C@@]3([H])CC4=C(C)[C@@]5(O[C@@]6([H])[C@@]([C@H]5C)([H])NC[C@@H](C)C6)CC[C@@]4([H])[C@]3([H])C1
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Synonyms
Ebeiensine
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Total alkaloids of Fritillaria unibracteata var. wabuensis bulbus ameliorate chronic asthma via the TRPV1/Ca2+/NFAT pathway. [Abstract]2023 Sep:118:154946. PMID: 37421766 -
Molecules
Metabolite Profiling and Anti-Inflammatory Activities of Fritillaria cirrhosa D. Don Bulbs Derived from Tissue Culture. [Abstract]2025 Jan 31;30(3):623. PMID: 39942727 -
J Pharm Pharmacol
Peimisine ameliorates DSS-induced colitis by suppressing Jak-Stat activation and alleviating gut microbiota dysbiosis in mice. [Abstract]2024 May 3;76(5):545-558. PMID: 38007392
Peimisine purchased from MedChemExpress. Usage Cited in: J Pharm Pharmacol. 2024 May 3;76(5):545-558. [Abstract]
Colon length treated with Peimisine (5, 15 mg/kg).
Peimisine purchased from MedChemExpress. Usage Cited in: J Pharm Pharmacol. 2024 May 3;76(5):545-558. [Abstract]
Representative images of HE staining of colon tissues and histological scores based on HE-stained sections treated with Peimisine (5, 15 mg/kg).
Peimisine purchased from MedChemExpress. Usage Cited in: J Pharm Pharmacol. 2024 May 3;76(5):545-558. [Abstract]
Western-blot analysis of IL-17A, FoxP3, and IL-17A/FoxP3 expression in mice colon treated with Peimisine (5, 15 mg/kg).
Peimisine purchased from MedChemExpress. Usage Cited in: J Pharm Pharmacol. 2024 May 3;76(5):545-558. [Abstract]
Immunofluorescence assessment of intestinal IL-17 (red) and FoxP3 (green) treated with Peimisine (5, 15 mg/kg).
Peimisine purchased from MedChemExpress. Usage Cited in: J Pharm Pharmacol. 2024 May 3;76(5):545-558. [Abstract]
Expression levels of inflammatory cytokines MCP-1, TNF-α, IL-6, IL-1β, and IFN-γ in serum treated with Peimisine (5, 15 mg/kg).
용액&용해도
In Vitro:
DMSO : 10 mg/mL (23.39 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (2.34 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (2.34 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
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Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
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TUNEL staining for apoptotic DNA fragmentation
TUNEL staining detects DNA strand breaks by using terminal deoxynucleotidyl transferase to add labeled nucleotides to exposed 3′-OH DNA termini, generating either microscopic staining in fixed cells or tissue sections, or fluorescence/cytometric signal in cell suspensions. TUNEL positivity reflects DNA fragmentation but should not be interpreted alone as definitive apoptosis, because TUNEL can also label necrotic, autolytic, mechanically damaged, or DNA-repair-associated DNA breaks.
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Annexin V plus membrane-impermeant dye apoptosis staining
Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis. Fluorescently labeled Annexin V binds PS in a calcium-dependent manner, enabling identification of early apoptotic cells by flow cytometry or fluorescence microscopy. When combined with a membrane-impermeant DNA-binding dye (e. g. , propidium iodide), this approach allows discrimination between viable (Annexin V−/dye−), early apoptotic (Annexin V+/dye−), and late apoptotic or necrotic (Annexin V+/dye+) cell populations by assessing membrane integrity and PS exposure.
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Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
순도&문서
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Data Sheet (277 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Pan F, et al. Peimisine and peiminine production by endophytic fungus Fusarium sp. isolated from Fritillaria unibracteata var. wabensis. Phytomedicine. 2014 Jul-Aug;21(8-9):1104-9. [Content Brief]
[2]. Armando Alberola-Die, et al. Peimine, an Anti-Inflammatory Compound from Chinese Herbal Extracts, Modulates Muscle-Type Nicotinic Receptors. Int J Mol Sci. 2021 Oct 19;22(20):11287. [Content Brief]
[3]. Dongdong Wang, et al. Evaluation of antitumor property of extracts and steroidal alkaloids from the cultivated Bulbus Fritillariae ussuriensis and preliminary investigation of its mechanism of action. BMC Complement Altern Med. 2015 Feb 21;15:29. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.3385 mL | 11.6926 mL | 23.3852 mL | 58.4631 mL |
| 5 mM | 0.4677 mL | 2.3385 mL | 4.6770 mL | 11.6926 mL | |
| 10 mM | 0.2339 mL | 1.1693 mL | 2.3385 mL | 5.8463 mL | |
| 15 mM | 0.1559 mL | 0.7795 mL | 1.5590 mL | 3.8975 mL | |
| 20 mM | 0.1169 mL | 0.5846 mL | 1.1693 mL | 2.9232 mL |
Peimisine Related Classifications
HY-N0214 Related Classifications
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