PMSF (100×)
Based on 1 Customer Validation
MCE PMSF (100×) is a ready-to-use protease inhibitor solution. It can be added directly to lysis buffers at a 1:100 dilution before use.
-
Storage :
-20°C，1 year. Avoid repeated freeze/thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
PMSF (Phenylmethylsulfonyl fluoride) is a non-specific and irreversible inhibitor of serine proteases. It can inhibit a variety of serine proteases and also exhibits inhibitory effects on certain thiol proteases, non-specific esterases, and acetylcholinesterase. PMSF is commonly used to inhibit protease activity during tissue or cell lysis, thereby protecting protein integrity. It is widely used in protein extraction and sample preparation procedures. The recommended final concentration of PMSF in lysis buffers is typically 0.5–1 mM.
MCE PMSF (100×) is a ready-to-use protease inhibitor solution. It can be added directly to lysis buffers at a 1:100 dilution before use.
Protocol
Allow the product to thaw at room temperature before use. Add PMSF to the lysis buffer at a 1:100 (v/v) dilution, mix well, and then proceed with sample lysis.
Note: PMSF is unstable in aqueous solutions and should be added to the lysis buffer immediately before use.
보관
-20°C，1 year. Avoid repeated freeze/thaw cycles.
주의
1. To avoid repeated freeze/thaw cycles that may affect product stability, it is recommended to aliquot the product into appropriate volumes according to experimental needs before storage.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.
Please enter the correct Lot Number
