NFYi5
Based on 1 Customer Validation
NFYi5 is a nuclear transcription factor-Y (NF-Y) inhibitor. NFYi5 disrupts the binding of NF-Y to DNA, accelerates the ubiquitin-independent degradation of the NF-YA subunit, and reduces the transcriptional activity of NF-Y. As an antimitotic agent, NFYi5 decreases the mRNA levels of NF-Y target genes without affecting the expression of housekeeping genes, and inhibits cell proliferation. NFYi5 can be used in the research of tissue fibrosis.
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- Purity : 97.37%
- CAS No.: 925644-66-2
- 화학식: C19H22N6O
- 분자량:350.42
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보관:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
More
Biological Activity
제품 설명
In Vitro
NFYi5 (0.01-100 μM; 18 h) inhibits NF-Y-dependent reporter gene activity in a dose-dependent manner, with an IC50 of 19.95 μM in human cardiac fibroblasts and 12.73 μM in rat cardiac fibroblasts, while exerting no effect on non-NF-Y promoter activity[1].
NFYi5 (10-20 μM; 18 h) selectively reduces the mRNA levels of NF-Y target genes in human and rat cardiac fibroblasts without affecting the expression of housekeeping genes[1].
NFYi5 (5-20 μM; 24-48 h) inhibits the proliferation of human and rat cardiac fibroblasts in a dose-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human cardiac fibroblasts, rat cardiac fibroblasts
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Concentration:10, 20 μM
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Incubation Time:18 h
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Result:Reduced mRNA levels of NF-Y target genes CCNA2 and CCNB1 in human cardiac fibroblasts without altering GAPDH levels.
Dose-dependently reduced mRNA levels of NF-Y target genes CCNA2, CCNB1, ECT2, and DDX11 in rat cardiac fibroblasts, while leaving housekeeping gene mRNA levels (TBP, 36B4, GAPDH, UBC) unchanged.
- RNA extraction experimental
- Collagen: Sirius Red Staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- Connective Tissue: Masson's Trichrome/Collagen Trichrome Staining
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- Nuclear Protein Extraction (High-Salt/Hypotonic Fractionation)
- Fibrosis/Collagen Morphometry
- Dual Luciferin reporter gene assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- MTT Cell Proliferation Assay
Chemical Information
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CAS No. 925644-66-2
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Appearance Solid
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분자량 350.42
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화학식 C19H22N6O
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Color White to off-white
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SMILES
CC1=C2C(C(C)=NC(NC3=NCN(CN3)CC4=CC=CO4)=N2)=CC(C)=C1
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
In Vitro:
DMSO : 22.22 mg/mL (63.41 mM; ultrasonic and adjust pH to 2; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8537 mL | 14.2686 mL | 28.5372 mL | 71.3430 mL |
| 5 mM | 0.5707 mL | 2.8537 mL | 5.7074 mL | 14.2686 mL | |
| 10 mM | 0.2854 mL | 1.4269 mL | 2.8537 mL | 7.1343 mL | |
| 15 mM | 0.1902 mL | 0.9512 mL | 1.9025 mL | 4.7562 mL | |
| 20 mM | 0.1427 mL | 0.7134 mL | 1.4269 mL | 3.5671 mL | |
| 25 mM | 0.1141 mL | 0.5707 mL | 1.1415 mL | 2.8537 mL | |
| 30 mM | 0.0951 mL | 0.4756 mL | 0.9512 mL | 2.3781 mL | |
| 40 mM | 0.0713 mL | 0.3567 mL | 0.7134 mL | 1.7836 mL | |
| 50 mM | 0.0571 mL | 0.2854 mL | 0.5707 mL | 1.4269 mL | |
| 60 mM | 0.0476 mL | 0.2378 mL | 0.4756 mL | 1.1890 mL |
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