Ristomycin sulfate
Based on 1 Customer Validation
Ristomycin sulfate is an antibacterial antibiotic and platelet aggregation inducer. Ristomycin sulfate interacts with the D-alanyl-D-alanine terminus of bacterial cell wall precursors to regulate bacterial cell wall synthesis. Ristomycin sulfate induces platelet aggregation in vitro and inhibits the growth of Gram-positive bacteria. Ristomycin sulfate serves as an in vitro diagnostic compound for detecting von Willebrand factor activity. Ristomycin sulfate is applicable to research related to von Willebrand disease and Bernard-Soulier syndrome.
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- Purity: 99.0%
- CAS No.: 11140-99-1
- 화학식: C95H110N8O44.H2SO4
- 분자량:2166.00
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보관:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
All Antibiotic Isoforms
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Biological Activity
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Glycopeptide |
Ristomycin (sulfate) produced by recombinant A. japonicum/pRM4-bbr_Aba (20 μl culture supernatant; 5 days) strongly inhibits the growth of Bacillus subtilis ATCC 6633 in an agar diffusion assay after 5 days of production in R5 medium[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 11140-99-1
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Appearance Solid
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분자량 2166.00
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화학식 C95H110N8O44.H2SO4
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Color White to off-white
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SMILES
O=S(O)(O)=O.[Ristomycin]
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Structure Classification
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Initial Source
Nocardia lurida
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
DMSO : 100 mg/mL (46.17 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 100 mg/mL (46.17 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.15 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.15 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
순도&문서
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Data Sheet (278 KB)
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SDS (416 KB)
- English - EN (416 KB)
- Français - FR (416 KB)
- Deutsch - DE (416 KB)
- Norwegian - NO (416 KB)
- Español - ES (416 KB)
- Swedish - SV (416 KB)
- Italian - IT (416 KB)
- Portuguese - PT (416 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 0.4617 mL | 2.3084 mL | 4.6168 mL | 11.5420 mL |
| 5 mM | 0.0923 mL | 0.4617 mL | 0.9234 mL | 2.3084 mL | |
| 10 mM | 0.0462 mL | 0.2308 mL | 0.4617 mL | 1.1542 mL | |
| 15 mM | 0.0308 mL | 0.1539 mL | 0.3078 mL | 0.7695 mL | |
| 20 mM | 0.0231 mL | 0.1154 mL | 0.2308 mL | 0.5771 mL | |
| 25 mM | 0.0185 mL | 0.0923 mL | 0.1847 mL | 0.4617 mL | |
| 30 mM | 0.0154 mL | 0.0769 mL | 0.1539 mL | 0.3847 mL | |
| 40 mM | 0.0115 mL | 0.0577 mL | 0.1154 mL | 0.2886 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ristomycin sulfate Related Classifications
Cat. No.: HY-131150
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- Ristomycin
- 11140-99-1
- Antibiotic
- Bacterial
- von Willebrand disease
- Bernard-Soulier syndrome
- von Willebrand factor
- bacterial cell wall precursors
- bacterial cell wall synthesis
- A. japonicum/pRM4-bbr_Aba
- platelet agglutination
- Gram-positive bacteria
- Bacillus subtilis ATCC 6633
- D-alanyl-D-alanine terminus
- Inhibitor
- inhibitor
- inhibit