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Cat. No.: HY-P99175

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Biologically Validated

KWAR 23

Name: KWAR 23
Brand: MedChemExpress
SKU: HY-P99175
Price: 620.00 USD
Availability: InStock
Rating: 5 (1 reviews)

Customer Review

Based on 1 Customer Validation

KWAR23 is an anti-human SIRPα antibody. KWAR23 binds human SIRPα with high affinity and disrupts its binding to CD47. KWAR23 shows antitumor activity in combination with tumor-opsonizing antibodies and can be used in cancer immunotherapy research.

For research use only. We do not sell to patients.

Purity : 99.24%
CAS No.: 2243227-60-1
Molecular Weight:143.4 kDa

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

KWAR 23 Chemical Structure

CAS No.: 2243227-60-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- In Vivo
- Gene ID
- Accession
- Conjugated
- Reconsititution
- Format
- Application
- Verified Bioactivity
Chemical Information
Protocols (1)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Isotype

Human IgG4 kappa

Recommend Isotype Controls

Human IgG4 (S228P) kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

SIRPα

In Vitro

KWAR 23 (0-1 μM; 4 h) shows antitumor activity of human neutrophil and macrophage^[1].
KWAR 23 induces human macrophage-dependent phagocytosis in human tumor-derived cell lines^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Cell Viability Assay^[1]

View Protocol

Cell Line:

Human macrophages and lymphoma cells
Concentration:

0-1 μM
Incubation Time:

4 h
Result:

Showed human macrophages and neutrophils killing tumor cells following treatment with KWAR23.

In Vivo

KWAR23 (intraperitoneal injection; 10 mg/kg; every other day; 21 d) exhibits effective antitumor activity in a human SIRPA knockin mouse model^[1].
KWAR23 enhances neutrophil and macrophage antitumor activity in SRG Mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

SIRPA knock-in mouse model injected with Burkitt’s lymphoma cells^[1]
Dosage:

10 mg/kg
Administration:

Intraperitoneal injection; 10 mg/kg; every other day; 21 d
Result:

Led to strong inhibition of tumor growth when combined with Rituximab.

Gene ID

140885 [NCBI]

Accession

P78324

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Format

Human IgG4 kappa

Application

ELISA, FACS, Functional assay

Verified Bioactivity

Immobilized SIRP alpha/CD172a Protein, Human can bind KWAR 23. The EC₅₀ for this effect is 5.3 ng/mL.
Flow Cytometry analysis of U937 cells labelling SIRPα (red) with KWAR 23 (anti-SIRPα) (HY-P99175). Goat Anti-Human IgG (Alexa Fluor 488) (HY-P83776) at a dilution of 1/1000 was used as the secondary antibody. Blue-Human IgG4 kappa (HY-P99003). Black-Unlabelled control, cells without incubation with primary antibody.

Chemical Information

CAS No. 2243227-60-1
Appearance Liquid
Molecular Weight 143.4 kDa
Color Colorless to light yellow
SMILES

[KWAR 23]
Shipping

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Animals and Diseases

Subcutaneous Cell-Line-Derived Xenograft
Subcutaneous cell-line-derived xenograft (CDX) models are established by implanting cultured human cancer cell lines into immunodeficient mice, where the injected cells form localized tumors that can be monitored in vivo as a measure of tumorigenic potential, growth kinetics, and treatment response. These models are widely used in oncology research because they allow reproducible tumor formation and enable comparative assessment of tumor growth between different cell lines or genetic manipulations in a controlled in vivo microenvironment. Subcutaneous implantation of cancer cells in immunodeficient mice is a standard approach for evaluating tumor growth behavior and therapeutic response across multiple cancer types, including prostate, esophageal, pancreatic, and colon cancer models.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (260 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Inhibitory Antibodies User Guide (603 KB)

References

[1]. Ring NG, et al. Anti-SIRPα antibody immunotherapy enhances neutrophil and macrophage antitumor activity. Proc Natl Acad Sci U S A. 2017 Dec 5;114(49):E10578-E10585. [Content Brief]

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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Amount: USD 0.00

Biological Activity
- Description
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- In Vivo
- Gene ID
- Accession
- Conjugated
- Reconsititution
- Format
- Application
- Biological Activity
Chemical Information
Protocols
Purity & Documentation
References

1/16

Human IgG4 kappa
1/16

Immobilized SIRP alpha/CD172a Protein, Human can bind KWAR 23. The EC₅₀ for this effect is 5.3 ng/mL.
1/16

Flow Cytometry analysis of U937 cells labelling SIRPα (red) with KWAR 23 (anti-SIRPα) (HY-P99175). Goat Anti-Human IgG (Alexa Fluor 488) (HY-P83776) at a dilution of 1/1000 was used as the secondary antibody. Blue-Human IgG4 kappa (HY-P99003). Black-Unlabelled control, cells without incubation with primary antibody.
1/16

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KWAR 23

Protein A Agarose

HOLO Human Interleukin-2 (IL-2) Detection Kit

Bradford Protein Assay Kit

Cell-ATP Viability Detection Kit

DPBS Buffer (1×)

Cytotoxicity LDH Assay Kit

PBS Buffer (1×)