KWAR 23
Based on 1 Customer Validation
KWAR23 is an anti-human SIRPα antibody. KWAR23 binds human SIRPα with high affinity and disrupts its binding to CD47. KWAR23 shows antitumor activity in combination with tumor-opsonizing antibodies and can be used in cancer immunotherapy research.
For research use only. We do not sell to patients.
- Purity: 99.24%
- CAS No.: 2243227-60-1
- Molecular Weight:143.4 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Human IgG4 kappa
Human
SIRPα
KWAR 23 (0-1 μM; 4 h) shows antitumor activity of human neutrophil and macrophage[1].
KWAR 23 induces human macrophage-dependent phagocytosis in human tumor-derived cell lines[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human macrophages and lymphoma cells
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Concentration:0-1 μM
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Incubation Time:4 h
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Result:Showed human macrophages and neutrophils killing tumor cells following treatment with KWAR23.
KWAR23 enhances neutrophil and macrophage antitumor activity in SRG Mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:SIRPA knock-in mouse model injected with Burkitt’s lymphoma cells[1]
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Dosage:10 mg/kg
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Administration:Intraperitoneal injection; 10 mg/kg; every other day; 21 d
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Result:Led to strong inhibition of tumor growth when combined with Rituximab.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Human IgG4 kappa
ELISA, FACS, Functional assay
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Immobilized SIRP alpha/CD172a Protein, Human can bind KWAR 23. The EC50 for this effect is 5.3 ng/mL. -
Flow Cytometry analysis of U937 cells labelling SIRPα (red) with KWAR 23 (anti-SIRPα) (HY-P99175). Goat Anti-Human IgG (Alexa Fluor 488) (HY-P83776) at a dilution of 1/1000 was used as the secondary antibody. Blue-Human IgG4 kappa (HY-P99003). Black-Unlabelled control, cells without incubation with primary antibody.
Chemical Information
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CAS No. 2243227-60-1
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Appearance Liquid
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Molecular Weight 143.4 kDa
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Color Colorless to light yellow
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SMILES
[KWAR 23]
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (260 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)