Lactonic sophorolipid
Based on 1 Customer Validation
Lactonic sophorolipid is an apoptosis inducer and antimicrobial surfactant with antitumor activity. Lactonic sophorolipid regulates Bax/Bcl-gene expression through caspase-3/9 and induces apoptosis in tumor cells. Lactonic sophorolipid can disrupt cell membrane permeability and exert antibacterial effects (MIC for oral pathogens is 100-400 μg/mL). Lactonic sophorolipid promotes mitochondrial membrane potential depolarization, activates the intrinsic apoptotic pathway, and can synergize with antibiotics to enhance the antibacterial effect. Lactonic sophorolipid can be used in liver cancer research and the development of oral hygiene antibacterial agents[1][2][3].
For research use only. We do not sell to patients.
- Purity: 97.31%
- CAS No.: 148409-20-5
- Formula: C34H56O14
- Molecular Weight:688.80
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Anti-Infection Compound Library
HY-L002
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Apoptosis Compound Library
HY-L003
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Anti-Cancer Compound Library
HY-L025
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Lipid Compound Library
HY-L043
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Antibacterial Compound Library
HY-L049
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Antibiotics Library
HY-L067
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Anti-Liver Cancer Compound Library
HY-L101
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Heterocyclic Compound Library
HY-L138
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Cell Death Library
HY-L162
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MCE Bioactive Compound Library
HY-L001V
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
All Antibiotic Isoforms
More
Biological Activity
Lactonic sophorolipid inhibits HepG2 cell viability with IC50=25 μg/mL (24 h) and induces apoptosis, accompanied by increased caspase-3/9 enzyme activity and up-regulated Bax/Bcl-2 gene expression[1].
Lactonic sophorolipid (0.001-100 μg/mL; 24 h) inhibits proliferation in a dose-dependent manner in HT29, HCT116 and other colon cancer cells, and also has cytotoxic effects on normal cells[2].
Lactonic sophorolipid (100-400 μg/mL; 24-48 h) shows antibacterial activity against oral pathogens such as Streptococcus mutans and Actinomyces naeslundii (MIC=100-400 μg/mL), and disrupts biofilm formation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 148409-20-5
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Appearance Solid
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Molecular Weight 688.80
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Formula C34H56O14
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Color Off-white to yellow
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SMILES
O[C@@H]1[C@]2([H])[C@@](O[C@H](CCCCCC/C=C\CCCCCCCC(O[C@]3([H])[C@H](O[C@@]([C@@H]([C@H]3O)O)([H])O2)COC(C)=O)=O)C)([H])O[C@@H]([C@H]1O)COC(C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 200 mg/mL (290.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.02 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Xiao Wang, et al. Lactonic sophorolipid-induced apoptosis in human HepG2 cells through the Caspase-3 pathway. Appl Microbiol Biotechnol. 2021 Mar;105(5):2033-2042. [Content Brief]
[2]. Callaghan B, et al. Lactonic Sophorolipids Increase Tumor Burden in Apcmin+/-Mice. PLoS One. 2016 Jun 6;11(6):e0156845. [Content Brief]
[3]. M Elshikh, et al. Rhamnolipids and lactonic sophorolipids: natural antimicrobial surfactants for oral hygiene. J Appl Microbiol. 2017 Nov;123(5):1111-1123. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4518 mL | 7.2590 mL | 14.5180 mL | 36.2950 mL |
| 5 mM | 0.2904 mL | 1.4518 mL | 2.9036 mL | 7.2590 mL | |
| 10 mM | 0.1452 mL | 0.7259 mL | 1.4518 mL | 3.6295 mL | |
| 15 mM | 0.0968 mL | 0.4839 mL | 0.9679 mL | 2.4197 mL | |
| 20 mM | 0.0726 mL | 0.3630 mL | 0.7259 mL | 1.8148 mL | |
| 25 mM | 0.0581 mL | 0.2904 mL | 0.5807 mL | 1.4518 mL | |
| 30 mM | 0.0484 mL | 0.2420 mL | 0.4839 mL | 1.2098 mL | |
| 40 mM | 0.0363 mL | 0.1815 mL | 0.3630 mL | 0.9074 mL | |
| 50 mM | 0.0290 mL | 0.1452 mL | 0.2904 mL | 0.7259 mL | |
| 60 mM | 0.0242 mL | 0.1210 mL | 0.2420 mL | 0.6049 mL | |
| 80 mM | 0.0181 mL | 0.0907 mL | 0.1815 mL | 0.4537 mL | |
| 100 mM | 0.0145 mL | 0.0726 mL | 0.1452 mL | 0.3630 mL |
Lactonic sophorolipid Related Classifications
HY-137371 Related Classifications
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