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Cat. No.: HY-183119

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Legumain-IN-2

Legumain-IN-2 is a selective legumain inhibitor with a human legumain IC₅₀ of 0.9 nM. Legumain-IN-2 can be used for the research of breast cancer.

For research use only. We do not sell to patients.

CAS No.: 1609690-02-9
Formula: C₂₅H₂₈N₄O₅
Molecular Weight:464.51

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Legumain-IN-2 Chemical Structure

CAS No.: 1609690-02-9

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Legumain-IN-2 is a selective legumain inhibitor with a human legumain IC₅₀ of 0.9 nM. Legumain-IN-2 can be used for the research of breast cancer^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	100 nM Compound: 7u	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 24775305]

In Vitro

Legumain-IN-2 (Compound 7u) potently inhibits recombinant human Legumain with an IC₅₀ of 0.9 nM^[1].
Legumain-IN-2 (30 μM) is highly selective for Legumain, showing minimal to moderate inhibition of other tested cysteine proteases^[1].
Legumain-IN-2 (30 μM; 120 h) reduces cell count by >70% in MCF7 and T47D breast cancer cells, but has no effect on MCF10a and HME-1 normal breast cell viability under the same conditions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

human breast cancer cell lines MCF7, T47D; normal human breast cell lines MCF10a, HME-1
Concentration:

30 μM
Incubation Time:

120 h
Result:

Reduced cell count by >70% in MCF7 and T47D breast cancer cells compared to vehicle control.
Had no impact on cell viability in MCF10a and HME-1 normal breast cell lines.

Chemical Information

CAS No. 1609690-02-9
Molecular Weight 464.51
Formula C₂₅H₂₈N₄O₅
SMILES

NC(C[C@@H](C#N)NC([C@H]1N(CCC1)C(OCC2=CC=C(C3=CC(OCC)=CC=C3)C=C2)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Higgins C, et al. P3 SAR exploration of biphenyl carbamate based Legumain inhibitors. Bioorg Med Chem Lett. 2014;24(11):2521-2524. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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