Lipid C16-C
Lipid C16-C is an ionizable cationic lipid that can be used to prepare lipid nanoparticles (LNPs) for mRNA delivery. Lipid C16-C mediates ApoE-independent macropinocytosis-based mRNA delivery to macrophages, with endosomal acidification enabling endosomal escape and release of mRNA cargo into the cytosol.
For research use only. We do not sell to patients.
- CAS No.: 2639634-86-7
- Formula: C98H201N5O9
- Molecular Weight:1593.67
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Lipid C16-C (250-500 ng mRNA per 50k cells; 24 h) enables potent mRNA delivery to PMA-differentiated human THP-1 macrophages, with the optimized B15 formulation showing 13-fold enhanced delivery relative to the initial formulation[1].
The B15 formulation with Lipid C16-C (up to 1500 ng mRNA per 50k cells; 24 h) enables robust mRNA delivery to both GMCSF- and MCSF-derived primary human macrophages, with differential transfection profiles between the two models[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2639634-86-7
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Molecular Weight 1593.67
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Formula C98H201N5O9
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SMILES
OC(CCCCCCCCCCCCCC)CN(CCN1CCN(CCOCCOCCN(CC(O)CCCCCCCCCCCCCC)CC(O)CCCCCCCCCCCCCC)CC1)CCOCCOCCN(CC(O)CCCCCCCCCCCCCC)CC(O)CCCCCCCCCCCCCC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Lipid C16-C Related Classifications
HY-W1130452 Related Classifications
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