MAO-B-IN-7
MAO-B-IN-7 is a potent and blood-brain barrier permeable MAO-B and AChE inhibitor with IC50s of 41 nM, 87 nM and 0.3 μM for human AChE, electric eel AChE and MAO-B, respectively. MAO-B-IN-7 can effectively alleviate oxidative stress and neuroinflammatory damage.
For research use only. We do not sell to patients.
- CAS No.: 2851012-65-0
- Formula: C25H31NO4
- Molecular Weight:409.52
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
MAO-B 0.3 μM (IC50) |
hAChE 41 nM (IC50) |
In Vitro
MAO-B-IN-7 (compound 7d) can effectively interact with Cu2+ to form the corresponding complexes, and has good ability to inhibit ROS’s production induced by Cu2+[1].
MAO-B-IN-7 (10-100 μM; 24 hours) decreases the viability of PC-12 cells at 100 μM, but does not show obvious cytotoxicity at 10 and 25 μM[1].
MAO-B-IN-7 (4-25 μM; 24 hours) protects H2O2-induced PC-12 cells injury, and the cell viability are 80.2%, 71.1% and 56.3% at 25.0 μM, 10.0 μM and 4.0 μM, respectively.[1].
MAO-B-IN-7 (2.5 μM and 10 μM; 8 and 24 hours) inhibits ROS production induced by LPS in BV-2 cells in a dose-dependent manner; inhibits NO production with 30.4%, 43.5% and 58.9% at the concentrations of 0.5 μM, 2.5 μM and 10.0 μM, respectively; also inhibits TNF-α release with 32.7%, 47.8% and 63.2% at the concentrations of μM, 2.5 μM and 10.0 μM, respectively[1].
MAO-B-IN-7 exhibits the parallel artificial membrane permeation of 8.59 × 10-6 in vitro blood-brain barrier permeation study[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:PC-12[1]
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Concentration:10 μM, 25 μM, 100 μM
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Incubation Time:24 hours
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Result:Decreased the viability of PC-12 cells at 100 μM.
Chemical Information
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CAS No. 2851012-65-0
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Molecular Weight 409.52
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Formula C25H31NO4
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SMILES
CCCCC1OC(C2=CC(OCC3=CC(O)=C(C=C3)CN4CCCCC4)=CC=C21)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
MAO-B-IN-7 Related Classifications
HY-146762 Related Classifications
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