MAT-POS-e194df51-1
Based on 1 Customer Validation
MAT-POS-e194df51-1 is an orally active, non-covalent and non-peptide SARS-CoV-2 main protease (Mpro) inhibitor with an IC50 value of 37nM. MAT-POS-e194df51-1 is cytotoxic with EC50 values of 64 nM and 126 nM in A549-ACE2-TMPRSS2 cells and HeLa -ACE2 cells, respectively.
For research use only. We do not sell to patients.
- Purity: 98.45%
- CAS No.: 2765433-05-2
- Formula: C24H21ClN4O3S
- Molecular Weight:480.97
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
63.8 nM
Compound: MAT-POS-e194df51-1
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Antiproliferative activity against human A549 cells assessed as reduction in cell growth measured after 72 hrs by MTT assay
Antiproliferative activity against human A549 cells assessed as reduction in cell growth measured after 72 hrs by MTT assay
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[PMID: 37659195] |
| Calu-3 | IC50 |
1.15 μM
Compound: MAT-POS-e194df51-1
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Cytotoxicity against human Calu-3 cells assessed as reduction in cell viability upto 500 uM measured after 48 hrs by Hoechst 33342 staining based analysis
Cytotoxicity against human Calu-3 cells assessed as reduction in cell viability upto 500 uM measured after 48 hrs by Hoechst 33342 staining based analysis
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[PMID: 37659195] |
| HeLa | IC50 |
149 nM
Compound: MAT-POS-e194df51-1
|
Antiproliferative activity against human HeLa cells assessed as reduction in cell viability measured after 72 hrs by CCK8 assay
Antiproliferative activity against human HeLa cells assessed as reduction in cell viability measured after 72 hrs by CCK8 assay
|
[PMID: 37659195] |
Chemical Information
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CAS No. 2765433-05-2
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Appearance Solid
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Molecular Weight 480.97
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Formula C24H21ClN4O3S
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Color White to off-white
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SMILES
N#CC1(CC1)CS(=O)(N2CC3=C([C@@H](C2)C(NC4=C5C=CC=CC5=CN=C4)=O)C=C(C=C3)Cl)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (207.91 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.20 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0791 mL | 10.3957 mL | 20.7913 mL | 51.9783 mL |
| 5 mM | 0.4158 mL | 2.0791 mL | 4.1583 mL | 10.3957 mL | |
| 10 mM | 0.2079 mL | 1.0396 mL | 2.0791 mL | 5.1978 mL | |
| 15 mM | 0.1386 mL | 0.6930 mL | 1.3861 mL | 3.4652 mL | |
| 20 mM | 0.1040 mL | 0.5198 mL | 1.0396 mL | 2.5989 mL | |
| 25 mM | 0.0832 mL | 0.4158 mL | 0.8317 mL | 2.0791 mL | |
| 30 mM | 0.0693 mL | 0.3465 mL | 0.6930 mL | 1.7326 mL | |
| 40 mM | 0.0520 mL | 0.2599 mL | 0.5198 mL | 1.2995 mL | |
| 50 mM | 0.0416 mL | 0.2079 mL | 0.4158 mL | 1.0396 mL | |
| 60 mM | 0.0347 mL | 0.1733 mL | 0.3465 mL | 0.8663 mL | |
| 80 mM | 0.0260 mL | 0.1299 mL | 0.2599 mL | 0.6497 mL | |
| 100 mM | 0.0208 mL | 0.1040 mL | 0.2079 mL | 0.5198 mL |
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