MBD
Based on 1 Customer Validation
MBD (7-(p-Methoxybenzylamino)-4-nitrobenz-2,1,3-oxadiazole), a new fluorescent probe for protein and nucleoprotein conformation, is applied to bacterial ribosomes and to bovine trypsinogen and trypsin. MBD is strongly fluorescent upon binding to a hydrophobic area of a macromolecule.
For research use only. We do not sell to patients.
- Purity: 99.60%
- CAS No.: 33984-50-8
- Formula: C14H12N4O4
- Molecular Weight:300.27
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Chemical Information
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CAS No. 33984-50-8
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Appearance Solid
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Molecular Weight 300.27
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Formula C14H12N4O4
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Color Orange to red
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SMILES
O=[N+](C1=CC=C(NCC2=CC=C(OC)C=C2)C3=NON=C31)[O-]
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Synonyms
7-(p-Methoxybenzylamino)-4-nitrobenz-2,1,3-oxadiazole
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 25 mg/mL (83.26 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Kenner RA, et al. A new fluorescent probe for protein and nucleoprotein conformation. Binding of 7-(p-methoxybenzylamino)-4-nitrobenzoxadiazole to bovine trypsinogen and bacterial ribosomes. Biochemistry. 1971;10(24):4433-4440. [Content Brief]
[2]. Kasahara Y, et al. Formation of a conformationally changed C1r, a subcomponent of the first component of human complement, as an intermediate of its autoactivation reaction. FEBS Lett. 1982;141(1):128-131. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3303 mL | 16.6517 mL | 33.3034 mL | 83.2584 mL |
| 5 mM | 0.6661 mL | 3.3303 mL | 6.6607 mL | 16.6517 mL | |
| 10 mM | 0.3330 mL | 1.6652 mL | 3.3303 mL | 8.3258 mL | |
| 15 mM | 0.2220 mL | 1.1101 mL | 2.2202 mL | 5.5506 mL | |
| 20 mM | 0.1665 mL | 0.8326 mL | 1.6652 mL | 4.1629 mL | |
| 25 mM | 0.1332 mL | 0.6661 mL | 1.3321 mL | 3.3303 mL | |
| 30 mM | 0.1110 mL | 0.5551 mL | 1.1101 mL | 2.7753 mL | |
| 40 mM | 0.0833 mL | 0.4163 mL | 0.8326 mL | 2.0815 mL | |
| 50 mM | 0.0666 mL | 0.3330 mL | 0.6661 mL | 1.6652 mL | |
| 60 mM | 0.0555 mL | 0.2775 mL | 0.5551 mL | 1.3876 mL | |
| 80 mM | 0.0416 mL | 0.2081 mL | 0.4163 mL | 1.0407 mL |
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