2. Apoptosis Autophagy Epigenetics
  3. Apoptosis Autophagy Histone Acetyltransferase
  4. MC4033

MC4033 shows IC50s of 39.4 μM, 52.1 μM, 41 μM and 30.1 μM in HCT116, H1299, A549 and U937, respectively.
MC4033 (25, 50, 100, and 200 μM, 72 h) reduces the level of H4K16Ac in HT29 cells, suggesting its ability to inhibit KAT8 in cells.

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MC4033

MC4033 構造式

CAS 番号 : 28532-21-0

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 95 在庫あり
Solution
10 mM * 1 mL in DMSO USD 95 在庫あり
Solid
1 mg $40 在庫あり
5 mg $87 在庫あり
10 mg $140 在庫あり
25 mg $281 在庫あり
50 mg $450 在庫あり
100 mg $720 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

MC4033 関連抗体

Top Publications Citing Use of Products

Histone Acetyltransferase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
CBP/p300 GCN5/PCAF TIP60 MOZ/MORF HBO1

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製品説明

MC4033 shows IC50s of 39.4 μM, 52.1 μM, 41 μM and 30.1 μM in HCT116, H1299, A549 and U937, respectively[1].
MC4033 (25, 50, 100, and 200 μM, 72 h) reduces the level of H4K16Ac in HT29 cells, suggesting its ability to inhibit KAT8 in cells[1].
IC50 & Target

12.1μM (lysine acetyltransferase 8, KAT8)[1]
Cellular Effect
Cell Line Type Value Description References
A549 IC50
41 μM
Compound: 19; MC4033
Antiproliferative activity against human A549 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay
Antiproliferative activity against human A549 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay
[PMID: 37155735]
HCT-116 IC50
39.4 μM
Compound: 19; MC4033
Antiproliferative activity against human HCT-116 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay
Antiproliferative activity against human HCT-116 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay
[PMID: 37155735]
HT-29 IC50
>100 μM
Compound: 19; MC4033
Antiproliferative activity against human HT-29 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay
Antiproliferative activity against human HT-29 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay
[PMID: 37155735]
NCI-H1299 IC50
52.1 μM
Compound: 19; MC4033
Antiproliferative activity against human NCI-H1299 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay
Antiproliferative activity against human NCI-H1299 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay
[PMID: 37155735]
U-937 IC50
30.1 μM
Compound: 19; MC4033
Antiproliferative activity against human U-937 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay
Antiproliferative activity against human U-937 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay
[PMID: 37155735]
体外実験

MC4033 shows IC50s of 39.4 μM, 52.1 μM, 41 μM and 30.1 μM in HCT116, H1299, A549 and U937, respectively[1].
MC4033 (25, 50, 100, and 200 μM, 72 h) reduces the level of H4K16Ac in HT29 cells, suggesting its ability to inhibit KAT8 in cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MC4033 関連抗体

Cell Proliferation Assay[1]

Cell Line: HT29, HCT116, HeLa, H1299, A549, H460, MCF7, U937, and U251 cells
Concentration: 10, 25, 50, and 100 μM
Incubation Time: 72 h
Result: Displayed dose-dependent antiproliferative effects in HCT116, H1299, A549, and U937 cell lines.
The inhibition rate of cell proliferation was 70% at 50 μM and >80% at 100 μM in U937 cells.

Cell Cycle Analysis[1]

Cell Line: HT29, HCT116, and HeLa cells
Concentration: 50 μM and 100 μM
Incubation Time: 72 h
Result: Propidium iodide (PI) staining showed a slight increase in the percentage of cells with DNA hypodiploid peak, indicative of apoptosis.

RT-PCR[1]

Cell Line: HCT116 cells
Concentration: 100 μM
Incubation Time: 48h
Result: Reduced the mRNA levels of oncogenes UCP2.

Immunofluorescence[1]

Cell Line: HT29 cells
Concentration: 50 μM
Incubation Time: 24 h
Result: Reduced H4K16Ac signal intensity by 80%.

Western Blot Analysis[1]

Cell Line: HCT116 cells
Concentration: 0,10, 25, 50,100 μM
Incubation Time: 48 h
Result: Showed that the altered ratio of LC3-II/-I and the regulation of p62 autophagy markers indicated the activation of autophagy in HCT116 cells.

Apoptosis Analysis[1]

Cell Line: HCT116 cells
Concentration: 100 μM or 10 μM (MC4033/CQ)
Incubation Time: 72 h
Result: Showed that exposure of HCT116 cells to CQ increased the apoptotic effect of KAT8i.
分子量

295.29

分子式

C16H13N3O3
CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

CC1=NN(C2=CC=C(C=C2)C(O)=O)C(/C1=C\C3=CC=CN3)=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 25 mg/mL (84.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3865 mL 16.9325 mL 33.8650 mL
5 mM 0.6773 mL 3.3865 mL 6.7730 mL
10 mM 0.3387 mL 1.6933 mL 3.3865 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3865 mL 16.9325 mL 33.8650 mL 84.6625 mL
5 mM 0.6773 mL 3.3865 mL 6.7730 mL 16.9325 mL
10 mM 0.3387 mL 1.6933 mL 3.3865 mL 8.4663 mL
15 mM 0.2258 mL 1.1288 mL 2.2577 mL 5.6442 mL
20 mM 0.1693 mL 0.8466 mL 1.6933 mL 4.2331 mL
25 mM 0.1355 mL 0.6773 mL 1.3546 mL 3.3865 mL
30 mM 0.1129 mL 0.5644 mL 1.1288 mL 2.8221 mL
40 mM 0.0847 mL 0.4233 mL 0.8466 mL 2.1166 mL
50 mM 0.0677 mL 0.3387 mL 0.6773 mL 1.6933 mL
60 mM 0.0564 mL 0.2822 mL 0.5644 mL 1.4110 mL
80 mM 0.0423 mL 0.2117 mL 0.4233 mL 1.0583 mL
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MC4033 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MC403328532-21-0MC 4033MC-4033ApoptosisAutophagyHistone AcetyltransferaseHATsHATapoptosiscancerautophagyNSCLCAMLInhibitorinhibitorinhibit

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製品名:
MC4033
製品番号:
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