2. Academic Validation
  3. Synthesis of 7-aminocoumarin by Buchwald-Hartwig cross coupling for specific protein labeling in living cells

Synthesis of 7-aminocoumarin by Buchwald-Hartwig cross coupling for specific protein labeling in living cells

  • Chembiochem. 2011 Jan 3;12(1):65-70. doi: 10.1002/cbic.201000414.
Xin Jin 1,  Chayasith Uttamapinant,  Alice Y Ting
Affiliations

Affiliation

  • 1 Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge MA 02139, USA.
PMID: 21154801 DOI: 10.1002/cbic.201000414
Abstract

PRIME time: We report the synthesis of a pH-insensitive blue fluorophore, 7-aminocoumarin, by using palladium-catalyzed Buchwald–Hartwig cross coupling. 7-Aminocoumarin can be used to tag recombinant proteins on the cell surface and inside living cells through PRIME (probe incorporation mediated by enzymes), and unlike 7-hydroxycoumarin, can be visualized in acidic organelles such as endosomes.

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