Met-Gly-Pro-AMC
Based on 1 Customer Validation
Met-Gly-Pro-AMC is a fluorescent peptide substrate active against *Caenorhabditis elegans* DPF-3, hDPP4, and methionyl aminopeptidase 2 (MetAP2). Met-Gly-Pro-AMC undergoes cleavage via tripeptidyl peptidase activity to release a fluorescent product. Met-Gly-Pro-AMC is used to detect the activities of tripeptidyl peptidases and MetAP2 peptidases in in vitro enzyme activity assays.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 1926163-52-1
- Formula: C22H28N4O5S
- Molecular Weight:460.55
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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DPP-4 |
The fluorogenic tripeptide substrate Met-Gly-Pro-AMC (0.5-600 μM; 30 min) is robustly processed by recombinant human DPP4 (Km = 461.5 μM), moderately processed by recombinant C. elegans DPF-3 (Km = 245.6 μM), and not processed by recombinant human DPP9 or catalytically impaired DPF-3S784A[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1926163-52-1
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Appearance Solid
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Molecular Weight 460.55
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Formula C22H28N4O5S
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Color White to off-white
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Sequence
Met-Gly-Pro-{AMC}
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Sequence Shortening
MGP-{AMC}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 50 mg/mL (108.57 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Trivedi A, et al. The Caenorhabditis elegans DPF-3 and human DPP4 have tripeptidyl peptidase activity. FEBS letters. 2026 Feb;600(3):324-333. [Content Brief]
[2]. Ng JY, et al. Tyrosine nitration moderates the peptidase activity of human methionyl aminopeptidase 2. Biochemical and biophysical research communications. 2013 Oct 11;440(1):37-42. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.1713 mL | 10.8566 mL | 21.7132 mL | 54.2829 mL |
| 5 mM | 0.4343 mL | 2.1713 mL | 4.3426 mL | 10.8566 mL | |
| 10 mM | 0.2171 mL | 1.0857 mL | 2.1713 mL | 5.4283 mL | |
| 15 mM | 0.1448 mL | 0.7238 mL | 1.4475 mL | 3.6189 mL | |
| 20 mM | 0.1086 mL | 0.5428 mL | 1.0857 mL | 2.7141 mL | |
| 25 mM | 0.0869 mL | 0.4343 mL | 0.8685 mL | 2.1713 mL | |
| 30 mM | 0.0724 mL | 0.3619 mL | 0.7238 mL | 1.8094 mL | |
| 40 mM | 0.0543 mL | 0.2714 mL | 0.5428 mL | 1.3571 mL | |
| 50 mM | 0.0434 mL | 0.2171 mL | 0.4343 mL | 1.0857 mL | |
| 60 mM | 0.0362 mL | 0.1809 mL | 0.3619 mL | 0.9047 mL | |
| 80 mM | 0.0271 mL | 0.1357 mL | 0.2714 mL | 0.6785 mL | |
| 100 mM | 0.0217 mL | 0.1086 mL | 0.2171 mL | 0.5428 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Met-Gly-Pro-AMC Related Classifications
HY-P4344 Related Classifications
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- Met-Gly-Pro-AMC
- 1926163-52-1
- MetAP
- Dipeptidyl Peptidase
- Fluorescent Dye
- recombinant C. elegans DPF-3
- recombinant human DPP4
- Caenorhabditis elegans DPF-3
- endothelial dysfunction
- DPF-3(S784A)
- tripeptidyl peptidase
- human DPP4
- methionyl aminopeptidase 2
- recombinant human MetAP2
- recombinant human DPP9
- Inhibitor
- inhibitor
- inhibit