MIRA-1
Based on 2 publication(s) in Google Scholar
MIRA-1 is a maleimide analogue. MIRA-1 can induce apoptosis in mutant p53 cells via restoration of p53-dependent transcriptional transactivation. MIRA-1 has anticancer activity.
For research use only. We do not sell to patients.
- Purity: 98.85%
- CAS No.: 72835-26-8
- Formula: C8H9NO4
- Molecular Weight:183.16
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Anti-Cancer Compound Library
HY-L025
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Autophagy Compound Library
HY-L029
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Anti-Aging Compound Library
HY-L034
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Covalent Screening Library
HY-L036
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Ferroptosis Compound Library
HY-L051
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Pyroptosis Compound Library
HY-L059
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Glutamine Metabolism Compound Library
HY-L064
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Anti-Pancreatic Cancer Compound Library
HY-L077
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Anti-Blood Cancer Compound Library
HY-L079
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Anti-Cancer Metabolism Compound Library
HY-L083
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Transcription Factor-Targeted Library
HY-L090
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Anti-Colorectal Cancer Compound Library
HY-L103
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Cysteine Targeted Covalent Library
HY-L153
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Cell Death Library
HY-L162
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Anti-Hematopathy Compound Library
HY-L171
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Anti-Ovarian Cancer Compound Library
HY-L173
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Covalent Screening Library Plus
HY-L036P
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Bioactive Compound Library
HY-L001
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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Cell Proliferation Compound Library
HY-L201
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) MIRA-1
More
Biological Activity
IC50: 10 μM (Saos-2 His273)[1]
MIRA-1 suppresses growth of Saos-2-His273 cells expressing mutant p53[1].
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MIRA-1 (25 μM; 48 hours) inhibits cell growth in a mutant p53-dependent manner[1].
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MIRA-1 (5 μM; 14 days) dramatically reduces the number of colonies formed by His273-expressing Saos-2 cells, but is much less efficient in inhibiting p53-null Saos-2 cells[1].
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MIRA-1 (10 μM; 48 hours) causes a substantial increase in the fraction of Saos-2 and Saos-2-His273 cells with a sub-G1 DNA content in the presence of mutant p53[1].
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MIRA-1 (5 and 10 μM; 24 hours) induces EGFP expression, MDM2 and Bax in SKOV-His175 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Saos-2-His273, H1299-His175, SKOV-His175 and SKOV-His273[1]
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Concentration:25 μM
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Incubation Time:48 hours
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Result:Inhibited cell growth in a mutant p53-dependent manner, with survival rate of 18.5~39% in no doxycycline and 71.5~87.5% in doxycycline.
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Cell Line:Saos-2 and Saos-2-His273 cells[1]
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Concentration:10 μM
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Incubation Time:48 hours
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Result:Caused a substantial increase in the fraction of cells with a sub-G1 DNA content in the presence of mutant p53.
Chemical Information
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CAS No. 72835-26-8
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Appearance Solid
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Molecular Weight 183.16
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Formula C8H9NO4
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Color White to light yellow
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SMILES
O=C(C=C1)N(COC(CC)=O)C1=O
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Synonyms
NSC 19630
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
2024 Dec 31:e2413368. PMID: 39737862 -
Biochem Pharmacol
WWOX-mediated p53/SAT1 and NRF2/FPN1 axis contribute to toosendanin-induced ferroptosis in hepatocellular carcinoma. [Abstract]2025 Jan 31:116790. PMID: 39894307
Solvent & Solubility
DMSO : 100 mg/mL (545.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (645 KB)
- English - EN (645 KB)
- Français - FR (645 KB)
- Deutsch - DE (645 KB)
- Norwegian - NO (645 KB)
- Español - ES (645 KB)
- Swedish - SV (645 KB)
- Italian - IT (645 KB)
- Korean - KR (645 KB)
- Portuguese - PT (645 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.4597 mL | 27.2985 mL | 54.5971 mL | 136.4927 mL |
| 5 mM | 1.0919 mL | 5.4597 mL | 10.9194 mL | 27.2985 mL | |
| 10 mM | 0.5460 mL | 2.7299 mL | 5.4597 mL | 13.6493 mL | |
| 15 mM | 0.3640 mL | 1.8199 mL | 3.6398 mL | 9.0995 mL | |
| 20 mM | 0.2730 mL | 1.3649 mL | 2.7299 mL | 6.8246 mL | |
| 25 mM | 0.2184 mL | 1.0919 mL | 2.1839 mL | 5.4597 mL | |
| 30 mM | 0.1820 mL | 0.9100 mL | 1.8199 mL | 4.5498 mL | |
| 40 mM | 0.1365 mL | 0.6825 mL | 1.3649 mL | 3.4123 mL | |
| 50 mM | 0.1092 mL | 0.5460 mL | 1.0919 mL | 2.7299 mL | |
| 60 mM | 0.0910 mL | 0.4550 mL | 0.9100 mL | 2.2749 mL | |
| 80 mM | 0.0682 mL | 0.3412 mL | 0.6825 mL | 1.7062 mL | |
| 100 mM | 0.0546 mL | 0.2730 mL | 0.5460 mL | 1.3649 mL |
MIRA-1 Related Classifications
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