1. Cell Cycle/DNA Damage Cytoskeleton Apoptosis
  2. Microtubule/Tubulin Apoptosis
  3. MPT0B014

MPT0B014  (Synonyms: 6-(3′,4′,5′-Trimethoxybenzoyl)quinoline)

Cat. No.: HY-120786 Purity: 99.67%
COA Handling Instructions

MPT0B014 is a tubulin polymerization inhibitor. MPT0B014 induces cancer cell apoptosis. MPT0B014 can be used for the research of cancer.

For research use only. We do not sell to patients.

MPT0B014 Chemical Structure

MPT0B014 Chemical Structure

CAS No. : 1215208-59-5

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 71 In-stock
Solution
10 mM * 1 mL in DMSO USD 71 In-stock
Solid
5 mg USD 65 In-stock
10 mg USD 110 In-stock
25 mg USD 230 In-stock
50 mg USD 390 In-stock
100 mg USD 650 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

MPT0B014 is a tubulin polymerization inhibitor. MPT0B014 induces cancer cell apoptosis. MPT0B014 can be used for the research of cancer[1].

IC50 & Target

Tubulin polymerization[1]

In Vitro

MPT0B014 (0-1 μM; 48 h) inhibits A549, H1299 and H226 cells growth in a dose-dependent manner[1].
MPT0B014 (0.05-0.3 μM; 24 and 48 h) arrests cell cycle at G2/M and sub-G1 phases and induces apoptosis in A549 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: A549, H1299, H226 and HUVEC cells
Concentration: 0, 0.025, 0.05, 0.075 and 1 μM
Incubation Time: 48 h
Result: Inhibited cell viability with IC50s of 0.109±0.01, 0.055±0.004, 0.077±0.005 and 0.536±0.166 μM against A549, H1299, H226 and HUVEC cells, respectively.

Cell Cycle Analysis[1]

Cell Line: A549, H1299 and H226
Concentration: 0.05, 0.1 and 0.3 μM
Incubation Time: 24 and 48 h
Result: Treatment for 24 h led to notable accumulation of cells in the G2/M phase. At 48 h, sub-G1 apoptotic cell populations were increased in a concentration-dependent manner. Cells in the G2/M phase began to rise at 12 h post-treatment and peaked at 24 h. Following this, there was an emergence of cells in the sub-G1 population phase until 48 h.

Western Blot Analysis[1]

Cell Line: A549, H1299 and H226
Concentration: 0.05, 0.1 and 0.3 μM
Incubation Time: 24 h
Result: Resulted in a marked increase in expression of the mitosis marker MPM2 and the proteins cyclin B1, Cdc2, Thr161, Aurora A and Aurora B in a concentration-dependent manner. Decreased the expression of Cdc (Tyr15) and Cdc25C, whereas total protein levels of Cdc2 did not change.

Apoptosis Analysis[1]

Cell Line: A549
Concentration: 0.05, 0.075, 0.1 and 0.3 μM
Incubation Time: 48 h
Result: Induced apoptosis in a concentration-dependent manner.

Western Blot Analysis[1]

Cell Line: A549
Concentration: 0.05, 0.1 and 0.3 μM
Incubation Time: 24, 36 and 48 h
Result: Induced activation of caspases-3, -7, -8 and -9, and cleavage of PARP in a time- and concentration-dependent manner. Significantly induced Bcl-2 phosphorylation. Down-regulated Mcl-1 expression in a concentration-dependent manner.
In Vivo

The combination of MPT0B014 (100 mg/kg; i.v./i.p.; daily for 25 days) and 25 mg/kg Erlotinib (HY-50896) significantly improves A549 tumor inhibition in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Nude athymic mice, A549 xenografts[1]
Dosage: 100 mg/kg alone or in combination with 25 mg/kg Erlotinib (HY-50896)
Administration: i.v./i.p., daily for 25 days
Result: The combined treatment resulted in more significant tumor growth delay (28%) compared with treatment alone (7%). The combination produced significantly higher anti-tumor activity. The growth of A549 cancer cell xenografts was suppressed by 11, 21 and 49% (tumor growth inhibition) after treatment with MPT0B014, Erlotinib and MPT0B014 plus Erlotinib, respectively.
Molecular Weight

323.34

Formula

C19H17NO4

CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=CC=C2N=CC=CC2=C1)C3=CC(OC)=C(OC)C(OC)=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (154.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0927 mL 15.4636 mL 30.9272 mL
5 mM 0.6185 mL 3.0927 mL 6.1854 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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In Vivo Dissolution Calculator
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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0927 mL 15.4636 mL 30.9272 mL 77.3180 mL
5 mM 0.6185 mL 3.0927 mL 6.1854 mL 15.4636 mL
10 mM 0.3093 mL 1.5464 mL 3.0927 mL 7.7318 mL
15 mM 0.2062 mL 1.0309 mL 2.0618 mL 5.1545 mL
20 mM 0.1546 mL 0.7732 mL 1.5464 mL 3.8659 mL
25 mM 0.1237 mL 0.6185 mL 1.2371 mL 3.0927 mL
30 mM 0.1031 mL 0.5155 mL 1.0309 mL 2.5773 mL
40 mM 0.0773 mL 0.3866 mL 0.7732 mL 1.9329 mL
50 mM 0.0619 mL 0.3093 mL 0.6185 mL 1.5464 mL
60 mM 0.0515 mL 0.2577 mL 0.5155 mL 1.2886 mL
80 mM 0.0387 mL 0.1933 mL 0.3866 mL 0.9665 mL
100 mM 0.0309 mL 0.1546 mL 0.3093 mL 0.7732 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MPT0B014
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