N-Acetyl-D-cysteine
Based on 3 publication(s) in Google Scholar
N-Acetyl-D-cysteine has antioxidant activities and scavenges ROS through the reaction with its thiol group, but cannot enter the glutathione metabolic pathway.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 26117-28-2
- Formula: C5H9NO3S
- Molecular Weight:163.20
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) N-Acetyl-D-cysteine
More
Customer Validation & Images
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Cell Imaging/Staining
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
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Bio/Physico-chemical Assay
Biological Activity
Description
In Vitro
N-Acetyl-D-cysteine (20 mM; 1 hour pretreatment; 12 hours) does not increase intracellular GSH levels, but GSH monoester does. D-NAC can not enhance hypoxic apoptosis. This demonstrate that GSH rather than D-NAC or NAC is responsible for enhancing hypoxic apoptosis[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 26117-28-2
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Appearance Solid
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Molecular Weight 163.20
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Formula C5H9NO3S
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Color White to off-white
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SMILES
SC[C@H](C(O)=O)NC(C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (3)
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Journal Impact Factor
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Most Recent
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Redox Biol
Dissecting the process of human neutrophil lineage determination by using alpha-lipoic acid inducing neutrophil deficiency model. [Abstract]2022 Aug;54:102392. PMID: 35797799 -
Cell Chem Biol
2025 May 15;32(5):767-775.e5. PMID: 40311609
N-Acetyl-D-cysteine purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2025 May 15;32(5):767-775.e5. [Abstract]
Cell morphology of WT Pfa1 and HT1080 cells cultured in normal media [Cys2(+)] or cystine-free [Cys2(-)] media in the presence or absence of 5 mM NAC, D-NAC (N-Acetyl-D-cysteine), or 1 μM Lip-1 for 24 or 72 h.
N-Acetyl-D-cysteine purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2025 May 15;32(5):767-775.e5. [Abstract]
Cell viability of WT Pfa1 and HT1080 cells maintained in normal media, and Pfa1 cells maintained in Cys2(-) media supplemented with 200 μM cystathionine treated with the indicated concentrations of L-buthionine sulfoximine (BSO) in the presence or absence of 5 mM NAC, D-NAC (N-Acetyl-D-cysteine), or 1 μM Lip-1 for 24 h.
N-Acetyl-D-cysteine purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2025 May 15;32(5):767-775.e5. [Abstract]
Cell morphology of GCL catalytic subunit knockout (GclcKO) Pfa1 cells cultured in the presence or absence of 5 mM NAC, D-NAC (N-Acetyl-D-cysteine), or 100 nM Lip-1 for 24 h.
N-Acetyl-D-cysteine purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2025 May 15;32(5):767-775.e5. [Abstract]
Intracellular GSH level of GclcKO Pfa1 cells cultured in the presence or absence of 5 mM NAC or D-NAC (N-Acetyl-D-cysteine) for 24 h. The cells were supplemented with 100 nM Lip-1 to prevent cell death. WT cells were used for normalization.
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Korean J Physiol Pharmacol
NOX4/Src regulates ANP secretion through activating ERK1/2 and Akt/GATA4 signaling in beating rat hypoxic atria. [Abstract]2021 Mar 1;25(2):159-166. PMID: 33602886
Solvent & Solubility
In Vitro:
H2O : 200 mg/mL (1225.49 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (612.75 mM); Clear solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Maika Deffieu, et al. Glutathione Participates in the Regulation of Mitophagy in Yeast.J Biol Chem. 2009 May 29;284(22):14828-37. [Content Brief]
[2]. B K Wong,et al. Selective Effects of N-acetylcysteine Stereoisomers on Hepatic Glutathione and Plasma Sulfate in Mice. Toxicol Appl Pharmacol [Content Brief]
[3]. Suparna Qanungo, et al. N-Acetyl-L-cysteine Enhances Apoptosis Through Inhibition of Nuclear factor-kappaB in Hypoxic Murine Embryonic Fibroblasts. J Biol Chem [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 6.1275 mL | 30.6373 mL | 61.2745 mL | 153.1863 mL |
| 5 mM | 1.2255 mL | 6.1275 mL | 12.2549 mL | 30.6373 mL | |
| 10 mM | 0.6127 mL | 3.0637 mL | 6.1275 mL | 15.3186 mL | |
| 15 mM | 0.4085 mL | 2.0425 mL | 4.0850 mL | 10.2124 mL | |
| 20 mM | 0.3064 mL | 1.5319 mL | 3.0637 mL | 7.6593 mL | |
| 25 mM | 0.2451 mL | 1.2255 mL | 2.4510 mL | 6.1275 mL | |
| 30 mM | 0.2042 mL | 1.0212 mL | 2.0425 mL | 5.1062 mL | |
| 40 mM | 0.1532 mL | 0.7659 mL | 1.5319 mL | 3.8297 mL | |
| 50 mM | 0.1225 mL | 0.6127 mL | 1.2255 mL | 3.0637 mL | |
| 60 mM | 0.1021 mL | 0.5106 mL | 1.0212 mL | 2.5531 mL | |
| 80 mM | 0.0766 mL | 0.3830 mL | 0.7659 mL | 1.9148 mL | |
| 100 mM | 0.0613 mL | 0.3064 mL | 0.6127 mL | 1.5319 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
N-Acetyl-D-cysteine Related Classifications
HY-136386 Related Classifications
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