N-Methylcytisine
Based on 2 publication(s) in Google Scholar
N-Methylcytisine (Caulophylline), a tricyclic quinolizidine alkaloid, exerts hypoglycaemic, analgesic and anti-inflammatory activities. N-methylcytisine is a selective ligand of nicotinic receptors of acetylcholine in the central nervous system and has a high affinity (Kd = 50 nM) to nicotinic acetylcholine receptors (nAChR) from squid optical ganglia.
For research use only. We do not sell to patients.
- Purity: 99.81%
- CAS No.: 486-86-2
- Formula: C12H16N2O
- Molecular Weight:204.27
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) N-Methylcytisine
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Biological Activity
kd: 50 nM (nicotinic acetylcholine receptors)[2]
N-Methylcytisine (16 mg/kg, p.o., once per day) significantly reduces the concentrations of TNF-α, IL-1β, and IL-6 as well as inhibiting the expression of p-NK-κB p65 in the colon tissue of mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:DSS-induced colitis ICR mouse model (18-22 g)[1]
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Dosage:4 and 16 mg/kg
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Administration:Intragastrically (i.g.) once a day
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Result:Markedly reduced the disease activity index score.
Evidently improved the symptom of DSS-induced colon shortening.
Ameliorated severe inflammation-induced damage exhibited in the DSS-induced colitis group.
Decreased the activity of myeloperoxidase in the colon.
Chemical Information
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CAS No. 486-86-2
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Appearance Solid
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Molecular Weight 204.27
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Formula C12H16N2O
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Color White to off-white
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SMILES
O=C1C=CC=C2N1C[C@]3([H])CN(C)C[C@@]2([H])C3
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Synonyms
Caulophylline
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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J Ethnopharmacol
Isoxanthohumol, a component of Sophora flavescens, promotes the activation of the NLRP3 inflammasome and induces idiosyncratic hepatotoxicity. [Abstract]2022 Mar 1:285:114796. PMID: 34740771 -
Int Immunopharmacol
Minimolide F alleviates inflammatory diseases by specifically targeting STING and blocking IRF3 recruitment. [Abstract]2026 May 1:176:116468. PMID: 41819671
Solvent & Solubility
DMSO : 120 mg/mL (587.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (489.54 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (10.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Jiao YF, et al. N-Methylcytisine Ameliorates Dextran-Sulfate-Sodium-Induced Colitis in Mice by Inhibiting the Inflammatory Response. Molecules. 2018 Feb 25;23(3). pii: E510. [Content Brief]
[2]. Pliashkevich IuG, et al. [N-methylcytisine--a selective ligand of nicotinic receptors of acetylcholine in the CNS]. Biull Eksp Biol Med. 1987 Dec;104(12):690-2. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.8954 mL | 24.4770 mL | 48.9541 mL | 122.3852 mL |
| 5 mM | 0.9791 mL | 4.8954 mL | 9.7908 mL | 24.4770 mL | |
| 10 mM | 0.4895 mL | 2.4477 mL | 4.8954 mL | 12.2385 mL | |
| 15 mM | 0.3264 mL | 1.6318 mL | 3.2636 mL | 8.1590 mL | |
| 20 mM | 0.2448 mL | 1.2239 mL | 2.4477 mL | 6.1193 mL | |
| 25 mM | 0.1958 mL | 0.9791 mL | 1.9582 mL | 4.8954 mL | |
| 30 mM | 0.1632 mL | 0.8159 mL | 1.6318 mL | 4.0795 mL | |
| 40 mM | 0.1224 mL | 0.6119 mL | 1.2239 mL | 3.0596 mL | |
| 50 mM | 0.0979 mL | 0.4895 mL | 0.9791 mL | 2.4477 mL | |
| 60 mM | 0.0816 mL | 0.4080 mL | 0.8159 mL | 2.0398 mL | |
| 80 mM | 0.0612 mL | 0.3060 mL | 0.6119 mL | 1.5298 mL | |
| 100 mM | 0.0490 mL | 0.2448 mL | 0.4895 mL | 1.2239 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
N-Methylcytisine Related Classifications
HY-N0443 Related Classifications
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