N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate
Based on 1 Customer Validation
N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate (TCFH) is a powerful coupling and derivatizing reagent for ester prodrug synthesis. N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate can be used for antitumor drugs before medicine research.
For research use only. We do not sell to patients.
- Purity : 99.63%
- CAS No.: 94790-35-9
- Formula: C5H12ClF6N2P
- Molecular Weight:280.58
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate (TCFH) mediates the synthesis of thiocoraline conjugates that maintain or slightly decrease antitumor activity in breast, non-small cell lung cancer (NSCLC, A-549), and colon (HT29) cell lines[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 94790-35-9
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Appearance Solid
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Molecular Weight 280.58
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Formula C5H12ClF6N2P
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Color White to off-white
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SMILES
C/[N+](C)=C(Cl)\N(C)C.F[P-](F)(F)(F)(F)F
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Synonyms
TCFH
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (356.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5640 mL | 17.8202 mL | 35.6405 mL | 89.1012 mL |
| 5 mM | 0.7128 mL | 3.5640 mL | 7.1281 mL | 17.8202 mL | |
| 10 mM | 0.3564 mL | 1.7820 mL | 3.5640 mL | 8.9101 mL | |
| 15 mM | 0.2376 mL | 1.1880 mL | 2.3760 mL | 5.9401 mL | |
| 20 mM | 0.1782 mL | 0.8910 mL | 1.7820 mL | 4.4551 mL | |
| 25 mM | 0.1426 mL | 0.7128 mL | 1.4256 mL | 3.5640 mL | |
| 30 mM | 0.1188 mL | 0.5940 mL | 1.1880 mL | 2.9700 mL | |
| 40 mM | 0.0891 mL | 0.4455 mL | 0.8910 mL | 2.2275 mL | |
| 50 mM | 0.0713 mL | 0.3564 mL | 0.7128 mL | 1.7820 mL | |
| 60 mM | 0.0594 mL | 0.2970 mL | 0.5940 mL | 1.4850 mL | |
| 80 mM | 0.0446 mL | 0.2228 mL | 0.4455 mL | 1.1138 mL | |
| 100 mM | 0.0356 mL | 0.1782 mL | 0.3564 mL | 0.8910 mL |
N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate Related Classifications
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