N-tert-Butyl-α-phenylnitrone
Based on 3 publication(s) in Google Scholar
N-tert-Butyl-α-phenylnitrone is a nitrone-based free radical scavenger that forms nitroxide spin adducts. N-tert-Butyl-α-phenylnitrone inhibits COX2 catalytic activity. N-tert-Butyl-α-phenylnitrone has potent ROS scavenging, anti-inflammatory, neuroprotective, anti-aging and anti-diabetic activities, and can penetrate the blood-brain barrier.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 3376-24-7
- Formula: C11H15NO
- Molecular Weight:177.24
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-N0361
Dihydrocapsaicin
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HY-D0221
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HY-N0361R
Dihydrocapsaicin (Standard)
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HY-W011757
Neocuproine hydrochloride monohydrate, 99%
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HY-W011757A
Neocuproine hydrochloride monohydrate, 95%
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![3,3'-Dimethyl-1,1'-diphenyl-1H,1'H-[4,4'-bipyrazole]-5,5'(4H,4'H)-dione (Standard)](https://file.medchemexpress.com/product_pic/hy-w009106r.gif)
HY-W009106R
3,3'-Dimethyl-1,1'-diphenyl-1H,1'H-[4,4'-bipyrazole]-5,5'(4H,4'H)-dione (Standard)
Publications Citing Use of MedChemExpress (MCE) N-tert-Butyl-α-phenylnitrone
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Biological Activity
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COX-2 |
Reactive oxygen species (ROS) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
10.8 mM
Compound: PBN
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Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
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[PMID: 27162124] |
| A549 | IC50 |
11.4 mM
Compound: PBN
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Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by FMCA assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by FMCA assay
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[PMID: 27162124] |
| A549 | IC50 |
9.6 mM
Compound: PBN
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Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by luminescence-based ATP assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by luminescence-based ATP assay
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[PMID: 27162124] |
| J774 | IC50 |
>400 μM
Compound: PBN
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Cytotoxicity against mouse J774 cells after 48 hrs by MTT assay
Cytotoxicity against mouse J774 cells after 48 hrs by MTT assay
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[PMID: 18788732] |
N-tert-Butyl-α-phenylnitrone (PBN) (25-100 μM) treatment leads to a significant decrease in 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced intracellular ROS accumulation. N-tert-Butyl-α-phenylnitrone also attenuates AAPH-induced cytotoxicity, matrix degradation, and apoptosis. N-tert-Butyl-α-phenylnitrone suppresses AAPH-induced activation of ERK/MAPK pathway. N-tert-Butyl-α-phenylnitrone has the potenial for intervertebral disc degeneration (IDD) research[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57Bl/6 mice induced by lipopolysaccharide (LPS)[2]
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Dosage:100 mg/kg
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Administration:Intraperitoneal injection; twice a day (on gestational day 8)
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Result:Abolished LPS-induced lipid peroxidation, nitrotyrosine residues, and GSH depletion.
Chemical Information
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CAS No. 3376-24-7
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Appearance Solid
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Molecular Weight 177.24
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Formula C11H15NO
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Color White to off-white
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SMILES
CC(/[N+]([O-])=C/C1=CC=CC=C1)(C)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Antioxidants (Basel)
Dopamine D2 Receptor Activation Blocks GluA2/ROS Positive Feedback Loop to Alienate Chronic-Migraine-Associated Pain Sensitization. [Abstract]2024 Jun 14;13(6):725. PMID: 38929165 -
Phytother Res
Astragaloside IV Relieves Central Sensitization by Regulating Astrocytic ROS/NF-κB Nuclear Translocation Signaling in Chronic Migraine Male Rats. [Abstract]2025 Mar;39(3):1438-1452. PMID: 39801364 -
Solvent & Solubility
DMSO : 100 mg/mL (564.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhenggang Zhou, et al. PBN Protects NP Cells From AAPH-induced Degenerative Changes by Inhibiting the ERK1/2 Pathway. Connect Tissue Res. 2020 Mar 30;1-10. [Content Brief]
[2]. Lei Zhao, et al. Reactive Oxygen Species Contribute to Lipopolysaccharide-Induced Teratogenesis in Mice. Toxicol Sci. 2008 May;103(1):149-57. [Content Brief]
[3]. Y Kotake, et al. Inhibition of NF-kappaB, iNOS mRNA, COX2 mRNA, and COX Catalytic Activity by phenyl-N-tert-butylnitrone (PBN). Biochim Biophys Acta. 1998 Nov 19;1448(1):77-84. [Content Brief]
[4]. R A Floyd. Antioxidants, Oxidative Stress, and Degenerative Neurological Disorders. Proc Soc Exp Biol Med. 1999 Dec;222(3):236-45. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.6421 mL | 28.2103 mL | 56.4207 mL | 141.0517 mL |
| 5 mM | 1.1284 mL | 5.6421 mL | 11.2841 mL | 28.2103 mL | |
| 10 mM | 0.5642 mL | 2.8210 mL | 5.6421 mL | 14.1052 mL | |
| 15 mM | 0.3761 mL | 1.8807 mL | 3.7614 mL | 9.4034 mL | |
| 20 mM | 0.2821 mL | 1.4105 mL | 2.8210 mL | 7.0526 mL | |
| 25 mM | 0.2257 mL | 1.1284 mL | 2.2568 mL | 5.6421 mL | |
| 30 mM | 0.1881 mL | 0.9403 mL | 1.8807 mL | 4.7017 mL | |
| 40 mM | 0.1411 mL | 0.7053 mL | 1.4105 mL | 3.5263 mL | |
| 50 mM | 0.1128 mL | 0.5642 mL | 1.1284 mL | 2.8210 mL | |
| 60 mM | 0.0940 mL | 0.4702 mL | 0.9403 mL | 2.3509 mL | |
| 80 mM | 0.0705 mL | 0.3526 mL | 0.7053 mL | 1.7631 mL | |
| 100 mM | 0.0564 mL | 0.2821 mL | 0.5642 mL | 1.4105 mL |
N-tert-Butyl-α-phenylnitrone Related Classifications
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