1. Metabolic Enzyme/Protease
    Anti-infection
    Cell Cycle/DNA Damage
  2. Endogenous Metabolite
    Enterovirus
    HCV
    Topoisomerase
  3. NHC-triphosphate tetrasodium

NHC-triphosphate tetrasodium 

Cat. No.: HY-135867A Purity: 98.06%
Handling Instructions

NHC-triphosphate tetrasodium is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a triphosphate form. NHC-triphosphate tetrasodium is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA.

For research use only. We do not sell to patients.

NHC-triphosphate tetrasodium Chemical Structure

NHC-triphosphate tetrasodium Chemical Structure

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Description

NHC-triphosphate tetrasodium is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a triphosphate form[1]. NHC-triphosphate tetrasodium is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA[1][2].

In Vitro

In an intracellular metabolism assay, HCV replicon cells are treated with 10 μM 3H-labeled NHC, and intracellular nucleotide levels are determined after 1, 2 and 8 hours incubations. NHC is rapidly convered into the mono-, di-, and triphosphate forms, and NHC-TP reaches up to 71.12 pM after 8 hours[1].
NHC-triphosphate (NHC-TP) (5-40 μM) absence leads to full-length polymerization products, it can be a weak alternative substrate. In addition, incorporation of NHC-TP instead of CTP increases the molecular weight of the polymerization product by 16 (one extra oxygen) for each event and an obvious electrophoretic shift is observed in cell-free HCV NS5B polymerization reactions[1].
Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC. Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP) can be observed, while NHC-triphosphate (HY-135867) remains the most abundant metabolite[2].
NHC-triphosphate (NHC-TP) metabolite may directly target the viral polymerase and behave as a nonobligate chain terminator. It plays a prominent role in inhibiting early negative-strand RNA synthesis, either through chain termination or mutagenesis, which may in turn interfere with correct replicase complex formation.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

587.08

Formula

C₉H₁₂N₃Na₄O₁₅P₃

SMILES

O[[email protected]]1[[email protected]](N(C=C/C2=N/O)C(N2)=O)O[[email protected]](COP(OP(OP(O[Na])(O[Na])=O)(O[Na])=O)(O[Na])=O)[[email protected]]1O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (170.33 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7033 mL 8.5167 mL 17.0335 mL
5 mM 0.3407 mL 1.7033 mL 3.4067 mL
10 mM 0.1703 mL 0.8517 mL 1.7033 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Keywords:

NHC-triphosphate tetrasodiumEndogenous MetaboliteEnterovirusHCVTopoisomeraseRhinovirusHRVHRVsHEVHEVsHepatitis C virusEnterovirus,VEEVCHIKVvenezuelanequineencephalitisChikungunyaintracellularmetaboliteInhibitorinhibitorinhibit

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NHC-triphosphate tetrasodium
Cat. No.:
HY-135867A
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