NHC-triphosphate tetrasodium
Based on 11 publication(s) in Google Scholar
NHC-triphosphate tetrasodium is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a triphosphate form. NHC-triphosphate tetrasodium is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA.
For research use only. We do not sell to patients.
- Formula: C9H12N3Na4O15P3
- Molecular Weight:587.08
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) NHC-triphosphate tetrasodium
More- Nucleic Acids Res. 2021 Jan 8;49(D1):D1113-D1121. [Abstract]
- J Control Release. 2025 Nov 10:387:114245. [Abstract]
- ACS Sens. 2022 May 27;7(5):1564-1571. [Abstract]
- Nat Struct Mol Biol. 2021 Sep;28(9):740-746. [Abstract]
- Commun Biol. 2022 Feb 22;5(1):154. [Abstract]
- PLoS Pathog. 2026 May 29;22(5):e1014225. [Abstract]
- J Biol Chem. 2026 Mar 26:111409. [Abstract]
- J Biol Chem. 2021 Jul;297(1):100770. [Abstract]
- ACS Bio Med Chem Au. 2022 Oct 24;2(6):565-585. [Abstract]
- NAR Mol Med. 2025 Dec 3;3(1):ugaf041. [Abstract]
- bioRxiv. 2021 Jul 21:2021.07.21.453274. [Abstract]
All Endogenous Metabolite Isoforms
MoreAll Topoisomerase Isoforms
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Biological Activity
In an intracellular metabolism assay, HCV replicon cells are treated with 10 μM 3H-labeled NHC, and intracellular nucleotide levels are determined after 1, 2 and 8 hours incubations. NHC is rapidly convered into the mono-, di-, and triphosphate forms, and NHC-TP reaches up to 71.12 pM after 8 hours[1].NHC-triphosphate (NHC-TP) (5-40 μM) absence leads to full-length polymerization products, it can be a weak alternative substrate. In addition, incorporation of NHC-TP instead of CTP increases the molecular weight of the polymerization product by 16 (one extra oxygen) for each event and an obvious electrophoretic shift is observed in cell-free HCV NS5B polymerization reactions[1].Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC. Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP) can be observed, while NHC-triphosphate (HY-135867) remains the most abundant metabolite[2].NHC-triphosphate (NHC-TP) metabolite may directly target the viral polymerase and behave as a nonobligate chain terminator. It plays a prominent role in inhibiting early negative-strand RNA synthesis, either through chain termination or mutagenesis, which may in turn interfere with correct replicase complex formation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 587.08
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Formula C9H12N3Na4O15P3
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Color White to off-white
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SMILES
O[C@H]1[C@H](N(C=C/C2=N/O)C(N2)=O)O[C@H](COP(OP(OP(O[Na])(O[Na])=O)(O[Na])=O)(O[Na])=O)[C@H]1O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (11)
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Journal Impact Factor
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Most Recent
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Nucleic Acids Res
COVID19 Drug Repository: text-mining the literature in search of putative COVID19 therapeutics. [Abstract]2021 Jan 8;49(D1):D1113-D1121. PMID: 33166390 -
J Control Release
A general nanoplatform for nucleotide drug delivery: From molecular binding to antiviral therapy. [Abstract]2025 Nov 10:387:114245. PMID: 40975176 -
ACS Sens
2022 May 27;7(5):1564-1571. PMID: 35427117 -
Nat Struct Mol Biol
2021 Sep;28(9):740-746. PMID: 34381216 -
Commun Biol
Combination of antiviral drugs inhibits SARS-CoV-2 polymerase and exonuclease and demonstrates COVID-19 therapeutic potential in viral cell culture. [Abstract]2022 Feb 22;5(1):154. PMID: 35194144 -
PLoS Pathog
2026 May 29;22(5):e1014225. PMID: 42213735 -
J Biol Chem
DNA-dependent RNA polymerase incorporates β-D-N4-hydroxycytidine (NHC) linking Molnupiravir to host transcription-dependent mutagenesis. [Abstract]2026 Mar 26:111409. PMID: 41903806 -
J Biol Chem
2021 Jul;297(1):100770. PMID: 33989635 -
ACS Bio Med Chem Au
Forodesine and Riboprine Exhibit Strong Anti-SARS-CoV-2 Repurposing Potential: In Silico and In Vitro Studies. [Abstract]2022 Oct 24;2(6):565-585. PMID: 37582236 -
NAR Mol Med
2025 Dec 3;3(1):ugaf041. PMID: 41509513 -
bioRxiv
Combination of Antiviral Drugs to Inhibit SARS-CoV-2 Polymerase and Exonuclease as Potential COVID-19 Therapeutics. [Abstract]2021 Jul 21:2021.07.21.453274. PMID: 34312622
Solvent & Solubility
H2O : 100 mg/mL (170.33 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Stuyver LJ,et al. Ribonucleoside analogue that blocks replication of bovine viral diarrhea and hepatitis C viruses in culture.Antimicrob Agents Chemother. 2003 Jan;47(1):244-54. [Content Brief]
[2]. Maryam Ehteshami, et al. Characterization of β-d- N4-Hydroxycytidine as a Novel Inhibitor of Chikungunya Virus. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.7033 mL | 8.5167 mL | 17.0335 mL | 42.5836 mL |
| 5 mM | 0.3407 mL | 1.7033 mL | 3.4067 mL | 8.5167 mL | |
| 10 mM | 0.1703 mL | 0.8517 mL | 1.7033 mL | 4.2584 mL | |
| 15 mM | 0.1136 mL | 0.5678 mL | 1.1356 mL | 2.8389 mL | |
| 20 mM | 0.0852 mL | 0.4258 mL | 0.8517 mL | 2.1292 mL | |
| 25 mM | 0.0681 mL | 0.3407 mL | 0.6813 mL | 1.7033 mL | |
| 30 mM | 0.0568 mL | 0.2839 mL | 0.5678 mL | 1.4195 mL | |
| 40 mM | 0.0426 mL | 0.2129 mL | 0.4258 mL | 1.0646 mL | |
| 50 mM | 0.0341 mL | 0.1703 mL | 0.3407 mL | 0.8517 mL | |
| 60 mM | 0.0284 mL | 0.1419 mL | 0.2839 mL | 0.7097 mL | |
| 80 mM | 0.0213 mL | 0.1065 mL | 0.2129 mL | 0.5323 mL | |
| 100 mM | 0.0170 mL | 0.0852 mL | 0.1703 mL | 0.4258 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
NHC-triphosphate tetrasodium Related Classifications
HY-135867A Related Classifications
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