NHC-triphosphate tetraammonium
Based on 11 publication(s) in Google Scholar
NHC-triphosphate tetraammonium is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a triphosphate form. NHC-triphosphate tetraammonium is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA.
For research use only. We do not sell to patients.
- Purity: 97.09%
- Formula: C9H28N7O15P3
- Molecular Weight:567.28
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Storage:
-80°C, protect from light, stored under nitrogen
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) NHC-triphosphate tetraammonium
More- Nucleic Acids Res. 2021 Jan 8;49(D1):D1113-D1121. [Abstract]
- J Control Release. 2025 Nov 10:387:114245. [Abstract]
- ACS Sens. 2022 May 27;7(5):1564-1571. [Abstract]
- Nat Struct Mol Biol. 2021 Sep;28(9):740-746. [Abstract]
- Commun Biol. 2022 Feb 22;5(1):154. [Abstract]
- PLoS Pathog. 2026 May 29;22(5):e1014225. [Abstract]
- J Biol Chem. 2026 Mar 26:111409. [Abstract]
- J Biol Chem. 2021 Jul;297(1):100770. [Abstract]
- ACS Bio Med Chem Au. 2022 Oct 24;2(6):565-585. [Abstract]
- NAR Mol Med. 2025 Dec 3;3(1):ugaf041. [Abstract]
- bioRxiv. 2021 Jul 21:2021.07.21.453274. [Abstract]
All Endogenous Metabolite Isoforms
MoreAll Topoisomerase Isoforms
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Biological Activity
In an intracellular metabolism assay, HCV replicon cells are treated with 10 μM 3H-labeled NHC, and intracellular nucleotide levels are determined after 1, 2 and 8 hours incubations. NHC is rapidly convered into the mono-, di-, and triphosphate forms, and NHC-TP reaches up to 71.12 pM after 8 hours[1].NHC-triphosphate tetraammonium (NHC-TP) (5-40 μM) absence leads to full-length polymerization products, it can be a weak alternative substrate. In addition, incorporation of NHC-TP instead of CTP increases the molecular weight of the polymerization product by 16 (one extra oxygen) for each event and an obvious electrophoretic shift is observed in cell-free HCV NS5B polymerization reactions[1].Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC. Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP) can be observed, while NHC-triphosphate tetraammonium (HY-135867) remains the most abundant metabolite[2].NHC-triphosphate tetraammonium (NHC-TP) metabolite may directly target the viral polymerase and behave as a nonobligate chain terminator. It plays a prominent role in inhibiting early negative-strand RNA synthesis, either through chain termination or mutagenesis, which may in turn interfere with correct replicase complex formation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 567.28
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Formula C9H28N7O15P3
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Color White to off-white
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SMILES
O[C@H]1[C@H](N(C=C/C2=N/O)C(N2)=O)O[C@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@H]1O.N.N.N.N
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Shipping
Shipping with dry ice.
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Storage
-80°C, protect from light, stored under nitrogen
Publications (11)
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Journal Impact Factor
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Most Recent
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Nucleic Acids Res
COVID19 Drug Repository: text-mining the literature in search of putative COVID19 therapeutics. [Abstract]2021 Jan 8;49(D1):D1113-D1121. PMID: 33166390 -
J Control Release
A general nanoplatform for nucleotide drug delivery: From molecular binding to antiviral therapy. [Abstract]2025 Nov 10:387:114245. PMID: 40975176 -
ACS Sens
2022 May 27;7(5):1564-1571. PMID: 35427117 -
Nat Struct Mol Biol
2021 Sep;28(9):740-746. PMID: 34381216 -
Commun Biol
Combination of antiviral drugs inhibits SARS-CoV-2 polymerase and exonuclease and demonstrates COVID-19 therapeutic potential in viral cell culture. [Abstract]2022 Feb 22;5(1):154. PMID: 35194144 -
PLoS Pathog
2026 May 29;22(5):e1014225. PMID: 42213735 -
J Biol Chem
DNA-dependent RNA polymerase incorporates β-D-N4-hydroxycytidine (NHC) linking Molnupiravir to host transcription-dependent mutagenesis. [Abstract]2026 Mar 26:111409. PMID: 41903806 -
J Biol Chem
2021 Jul;297(1):100770. PMID: 33989635 -
ACS Bio Med Chem Au
Forodesine and Riboprine Exhibit Strong Anti-SARS-CoV-2 Repurposing Potential: In Silico and In Vitro Studies. [Abstract]2022 Oct 24;2(6):565-585. PMID: 37582236 -
NAR Mol Med
2025 Dec 3;3(1):ugaf041. PMID: 41509513 -
bioRxiv
Combination of Antiviral Drugs to Inhibit SARS-CoV-2 Polymerase and Exonuclease as Potential COVID-19 Therapeutics. [Abstract]2021 Jul 21:2021.07.21.453274. PMID: 34312622
Solvent & Solubility
H2O : 100 mg/mL (176.28 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 4.25 mg/mL (7.49 mM); Clear solution
This protocol yields a clear solution of ≥ 4.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (42.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 4.25 mg/mL (7.49 mM); Clear solution
This protocol yields a clear solution of ≥ 4.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (42.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (176.28 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Stuyver LJ,et al. Ribonucleoside analogue that blocks replication of bovine viral diarrhea and hepatitis C viruses in culture.Antimicrob Agents Chemother. 2003 Jan;47(1):244-54. [Content Brief]
[2]. Maryam Ehteshami, et al. Characterization of β-d- N4-Hydroxycytidine as a Novel Inhibitor of Chikungunya Virus. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.7628 mL | 8.8140 mL | 17.6280 mL | 44.0699 mL |
| 5 mM | 0.3526 mL | 1.7628 mL | 3.5256 mL | 8.8140 mL | |
| 10 mM | 0.1763 mL | 0.8814 mL | 1.7628 mL | 4.4070 mL | |
| 15 mM | 0.1175 mL | 0.5876 mL | 1.1752 mL | 2.9380 mL | |
| 20 mM | 0.0881 mL | 0.4407 mL | 0.8814 mL | 2.2035 mL | |
| 25 mM | 0.0705 mL | 0.3526 mL | 0.7051 mL | 1.7628 mL | |
| 30 mM | 0.0588 mL | 0.2938 mL | 0.5876 mL | 1.4690 mL | |
| 40 mM | 0.0441 mL | 0.2203 mL | 0.4407 mL | 1.1017 mL | |
| 50 mM | 0.0353 mL | 0.1763 mL | 0.3526 mL | 0.8814 mL | |
| 60 mM | 0.0294 mL | 0.1469 mL | 0.2938 mL | 0.7345 mL | |
| 80 mM | 0.0220 mL | 0.1102 mL | 0.2203 mL | 0.5509 mL | |
| 100 mM | 0.0176 mL | 0.0881 mL | 0.1763 mL | 0.4407 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
NHC-triphosphate tetraammonium Related Classifications
HY-135867E Related Classifications
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