1. Metabolic Enzyme/Protease
    Anti-infection
    Cell Cycle/DNA Damage
  2. Endogenous Metabolite
    Enterovirus
    HCV
    Topoisomerase
    SARS-CoV
  3. NHC-triphosphate

NHC-triphosphate 

Cat. No.: HY-135867 Purity: 99.80%
Handling Instructions

NHC-triphosphate is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a triphosphate form. NHC-triphosphate is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA.

For research use only. We do not sell to patients.

NHC-triphosphate Chemical Structure

NHC-triphosphate Chemical Structure

CAS No. : 34973-27-8

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5 mg USD 800 In-stock
Estimated Time of Arrival: December 31
10 mg USD 1200 In-stock
Estimated Time of Arrival: December 31
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Based on 2 publication(s) in Google Scholar

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Description

NHC-triphosphate is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a triphosphate form[1]. NHC-triphosphate is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA[1][2].

In Vitro

In an intracellular metabolism assay, HCV replicon cells are treated with 10 μM 3H-labeled NHC, and intracellular nucleotide levels are determined after 1, 2 and 8 hours incubations. NHC is rapidly convered into the mono-, di-, and triphosphate forms, and NHC-TP reaches up to 71.12 pM after 8 hours[1].
NHC-triphosphate (NHC-TP) (5-40 μM) absence leads to full-length polymerization products, it can be a weak alternative substrate. In addition, incorporation of NHC-TP instead of CTP increases the molecular weight of the polymerization product by 16 (one extra oxygen) for each event and an obvious electrophoretic shift is observed in cell-free HCV NS5B polymerization reactions[1].
Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC. Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP) can be observed, while NHC-triphosphate (HY-135867) remains the most abundant metabolite[2].
NHC-triphosphate (NHC-TP) metabolite may directly target the viral polymerase and behave as a nonobligate chain terminator. It plays a prominent role in inhibiting early negative-strand RNA synthesis, either through chain termination or mutagenesis, which may in turn interfere with correct replicase complex formation.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

499.16

Formula

C₉H₁₆N₃O₁₅P₃

CAS No.
SMILES

O[[email protected]]1[[email protected]](N(C=C/C2=N/O)C(N2)=O)O[[email protected]](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[[email protected]]1O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 160 mg/mL (320.54 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0034 mL 10.0168 mL 20.0337 mL
5 mM 0.4007 mL 2.0034 mL 4.0067 mL
10 mM 0.2003 mL 1.0017 mL 2.0034 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: 99.80%

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Keywords:

NHC-triphosphateEndogenous MetaboliteEnterovirusHCVTopoisomeraseSARS-CoVRhinovirusHRVHRVsHEVHEVsHepatitis C virusSARS coronavirusEnterovirus,VEEVCHIKVvenezuelanequineencephalitisChikungunyaintracellularmetaboliteInhibitorinhibitorinhibit

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Product Name:
NHC-triphosphate
Cat. No.:
HY-135867
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