1. Cell Cycle/DNA Damage
    Anti-infection
  2. Topoisomerase
    HCV
  3. NHC-triphosphate

NHC-triphosphate 

Cat. No.: HY-135867
Handling Instructions

NHC-triphosphate is an intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) as a triphosphate form. NHC-triphosphate is a weak alternative substrate for the viral polymerase and changes the mobility of the product in polyacrylamide electrophoresis gels.

For research use only. We do not sell to patients.

NHC-triphosphate Chemical Structure

NHC-triphosphate Chemical Structure

CAS No. : 34973-27-8

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Description

NHC-triphosphate is an intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) as a triphosphate form. NHC-triphosphate is a weak alternative substrate for the viral polymerase and changes the mobility of the product in polyacrylamide electrophoresis gels[1].

IC50 & Target

IC50: alternative substrate for the viral polymerase[1]

In Vitro

In an intracellular metabolism assay, HCV replicon cells are treated with 10 μM 3H-labeled NHC, and intracellular nucleotide levels are determined after 1, 2 and 8 hours incubations. NHC is rapidly convered into the mono-, di-, and triphosphate forms, and NHC-TP reaches up to 71.12 pM after 8 hours[1].
NHC-triphosphate (NHC-TP)(5-40 μM) absence leads to full-length polymerization products, it can be a weak alternative substrate. In addition, incorporation of NHC-TP instead of CTP increases the molecular weight of the polymerization product by 16 (one extra oxygen) for each event and an obvious electrophoretic shift is observed in cell-free HCV NS5B polymerization reactions[1].

Molecular Weight

499.16

Formula

C₉H₁₆N₃O₁₅P₃

CAS No.

34973-27-8

SMILES

O[[email protected]]1[[email protected]](N(C=C/C2=N/O)C(N2)=O)O[[email protected]](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[[email protected]]1O

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

NHC-triphosphateTopoisomeraseHCVHepatitis C virusHepatitis CvirusmetabolitealternativesubstrateInhibitorinhibitorinhibit

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NHC-triphosphate
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