NHC-triphosphate tetrasodium

製品番号: HY-135867A: Data Sheet SDS 取扱説明書
FAQs
Technical Support

NHC-triphosphate tetrasodium is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a triphosphate form. NHC-triphosphate tetrasodium is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

NHC-triphosphate tetrasodium 構造式

容量	価格（税別）	在庫状況
1 mg	$360	見積もりとリードタイムを求めるのご確認
5 mg	$800	見積もりとリードタイムを求めるのご確認
10 mg	$1200	見積もりとリードタイムを求めるのご確認

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

Other 在庫あり Forms of NHC-triphosphate tetrasodium:

NHC-triphosphate tetraammonium

Other Forms of NHC-triphosphate tetrasodium:

NHC-diphosphate 在庫あり
NHC-triphosphate tetraammonium 在庫あり
NHC-triphosphate お問い合わせ

顧客検証

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Human Endogenous Metabolite Microbial Metabolite

Topoisomerase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Topoisomerase Topo I Topo II DNA gyrase

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

NHC-triphosphate tetrasodium is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a triphosphate form^[1]. NHC-triphosphate tetrasodium is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA^[1]^[2].

体外実験

In an intracellular metabolism assay, HCV replicon cells are treated with 10 μM ³H-labeled NHC, and intracellular nucleotide levels are determined after 1, 2 and 8 hours incubations. NHC is rapidly convered into the mono-, di-, and triphosphate forms, and NHC-TP reaches up to 71.12 pM after 8 hours^[1].
NHC-triphosphate (NHC-TP) (5-40 μM) absence leads to full-length polymerization products, it can be a weak alternative substrate. In addition, incorporation of NHC-TP instead of CTP increases the molecular weight of the polymerization product by 16 (one extra oxygen) for each event and an obvious electrophoretic shift is observed in cell-free HCV NS5B polymerization reactions^[1].
Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC. Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP) can be observed, while NHC-triphosphate (HY-135867) remains the most abundant metabolite^[2].
NHC-triphosphate (NHC-TP) metabolite may directly target the viral polymerase and behave as a nonobligate chain terminator. It plays a prominent role in inhibiting early negative-strand RNA synthesis, either through chain termination or mutagenesis, which may in turn interfere with correct replicase complex formation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

587.08

分子式

C₉H₁₂N₃Na₄O₁₅P₃

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@H](N(C=C/C2=N/O)C(N2)=O)O[C@H](COP(OP(OP(O[Na])(O[Na])=O)(O[Na])=O)(O[Na])=O)[C@H]1O

Structure Classification

Others

Initial Source

Endogenous metabolite

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

H₂O : 100 mg/mL (170.33 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7033 mL	8.5167 mL	17.0335 mL
5 mM	0.3407 mL	1.7033 mL	3.4067 mL
10 mM	0.1703 mL	0.8517 mL	1.7033 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

データシート (276 KB) SDS (251 KB) 取扱説明書 (2659 KB)

参考文献

[1]. Stuyver LJ,et al. Ribonucleoside analogue that blocks replication of bovine viral diarrhea and hepatitis C viruses in culture.Antimicrob Agents Chemother. 2003 Jan;47(1):244-54. [Content Brief]

[2]. Maryam Ehteshami, et al. Characterization of β-d- N4-Hydroxycytidine as a Novel Inhibitor of Chikungunya Virus. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.7033 mL	8.5167 mL	17.0335 mL	42.5836 mL
	5 mM	0.3407 mL	1.7033 mL	3.4067 mL	8.5167 mL
	10 mM	0.1703 mL	0.8517 mL	1.7033 mL	4.2584 mL
	15 mM	0.1136 mL	0.5678 mL	1.1356 mL	2.8389 mL
	20 mM	0.0852 mL	0.4258 mL	0.8517 mL	2.1292 mL
	25 mM	0.0681 mL	0.3407 mL	0.6813 mL	1.7033 mL
	30 mM	0.0568 mL	0.2839 mL	0.5678 mL	1.4195 mL
	40 mM	0.0426 mL	0.2129 mL	0.4258 mL	1.0646 mL
	50 mM	0.0341 mL	0.1703 mL	0.3407 mL	0.8517 mL
	60 mM	0.0284 mL	0.1419 mL	0.2839 mL	0.7097 mL
	80 mM	0.0213 mL	0.1065 mL	0.2129 mL	0.5323 mL
	100 mM	0.0170 mL	0.0852 mL	0.1703 mL	0.4258 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.