M918
M918 is a cell-penetrating peptide derived from the C-terminal region of the tumor suppressor p14ARF. Containing multiple arginine residues, M918 can bind to protein cargo and antisense PNA. By facilitating the delivery of sequence-specific antisense PNA, M918 inhibits and corrects splicing-thereby silencing luciferase expression-while simultaneously inducing Th1-type immune responses, including the production of IFN-γ, IgG2a, IgG2b, and Granzyme B. The intracellular internalization of M918 is independent of glycosaminoglycans; it exhibits low cytotoxicity and can be utilized in research concerning HIV-1 infection and related delivery strategies.
For research use only. We do not sell to patients.
- CAS No.: 955937-87-8
- Formula: C116H208N44O23S2
- Molecular Weight:2651.30
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
M918 (1 µmol/l; 30 min) partially co-localizes with both transferrin and dextran in MCF-7, CHO, and Hifko cells, but not with transferrin in HeLa cells[5].
M918 internalization is independent of glycosaminoglycans on the cell surface in CHO cells[5].
M918-PNA (5 µmol/l; 1 h) induces splice correction dose-dependently in HeLa pLuc 705 cells, with a 15-fold increase at 5 µmol/l[5].
M918 forms stable, non-covalent nanoparticles with Nef and Hsp20-Nef proteins; these nanoparticles enter cells via clathrin-mediated endocytosis and macropinocytosis, and exhibit robust endosomal escape and protein transduction capabilities[3].
M918 (5-25 µmol/l; 72 h) does not significantly affect cellular proliferation after 72 hours in any tested cell line[5].
M918 (1 µmol/l; 30 min) displays mainly vesicular localization inside MCF-7, Hifko, and HeLa cells[5].
M918 (1-5 µmol/l) is the most efficient vector for delivering streptavidin and avidin into cells when utilizing biotinylated peptides at 1 µmol/l, and it also promotes uptake of streptavidin at 5 µmol/l as a non-covalent complex[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT-29-luc cells
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Concentration:3-12 μM
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Incubation Time:10 h
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Result:Evaluated the relative cell viability at 3, 6, and 12 μM.
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Cell Line:HEK-293T cells
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Concentration:M918 peptide at 2, 5, 10, 15, 20, and 30 μM; Hsp20-Nef/M918 and Nef/M918 complexes at molar ratios of 2:1, 5:1, 10:1, 15:1, 20:1, and 30:1
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Incubation Time:48 h
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Result:Showed cell viability of 90-97% compared to untreated cells over a period of 48 h.
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Cell Line:HEK-293T cells
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Concentration:Molar ratio of 20:1 (protein:peptide); 100 μl applied per well
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Incubation Time:1 h at 37°C, then further incubated for 3 h
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Result:Detected dominant bands of ~27 kDa and ~47 kDa in the transfected cells with M918/Nef and M918/Hsp20-Nef nanoparticles using the anti-HIV Nef monoclonal antibody at 3 h post-transfection.
Did not reveal these bands in the un-transfected cells.
Did not detect any Nef/Hsp20-Nef-related bands in the transfected cells with Nef or Hsp20-Nef proteins alone.
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Cell Line:Several cell lines
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Concentration:5-25 µmol/l
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Incubation Time:72 h
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Result:No significant effect on proliferation after 72 hours was observed in any cell line.
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Cell Line:MCF-7, Hifko, HeLa
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Concentration:1 µmol/l
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Incubation Time:30 min
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Result:In MCF-7 and HeLa cells, no clusters of peptides were detected in the plasma membrane.
In Hifko cells, some membrane-associated peptides were observed.
Most internalized material appeared to reside in vesicles, with low or negligible amounts in the cell nucleus.
In HeLa cells, a slight diffuse staining of the cytoplasm was observed.
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Cell Line:MCF-7, CHO, Hifko
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Concentration:1 µmol/l
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Incubation Time:30 min
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Result:Localizes with both dextran and transferrin markers in MCF-7 cells.
Similar results were obtained in CHO and Hifko cells, whereas little localization was observed with transferrin in HeLa cells.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (female, 5-7 weeks old)[2]
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Dosage:5 μg protein with M918 at 1:20 molar ratio
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Administration:s.c.; days 0, 14, and 28
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Result:Induced the highest level of IFN-γ secretion in the heterologous pcDNA-Hsp20-Nef+MPG/rHsp20-Nef+M918 (G13) regimen.
Elicited higher IgG2a levels in homologous protein prime/protein boost and heterologous DNA prime/protein boost groups (G7, G10, G12, G13).
Induced significantly different IgG2b levels in the heterologous pcDNA-Hsp20-Nef+MPG/rHsp20-Nef+M918 (G13) group.
Stimulated significantly higher Granzyme B secretion in groups receiving M918 formulations.
Induced higher Granzyme B levels in the G13 group.
Chemical Information
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CAS No. 955937-87-8
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Molecular Weight 2651.30
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Formula C116H208N44O23S2
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Sequence
Met-Val-Thr-Val-Leu-Phe-Arg-Arg-Leu-Arg-Ile-Arg-Arg-Ala-Cys-Gly-Pro-Pro-Arg-Val-Arg-Val-NH2
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Sequence Shortening
MVTVLFRRLRIRRACGPPRVRV-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
M918 Related Classifications
HY-P10560 Related Classifications
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