Nurr1 agonist 5
Nurr1 agonist 5 (compound 5o) is a neuroprotective transcription factor Nurr1 agonist with a Kd of 0.5 μM and an EC50 of 3 μM.
For research use only. We do not sell to patients.
- CAS No.: 3033875-32-7
- Formula: C19H19Cl2N3O2
- Molecular Weight:392.28
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Nuclear Hormone Receptor 4A/NR4A Isoforms
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Biological Activity
Description
IC50 & Target
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Nurr1/NR4A2 |
Chemical Information
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CAS No. 3033875-32-7
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Molecular Weight 392.28
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Formula C19H19Cl2N3O2
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SMILES
ClC1=C(C=C2C(C=CN2)=C1)C(NC3=C(C=CC(Cl)=C3)OCCN(C)C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Nuclear Protein Extraction (High-Salt/Hypotonic Fractionation)
The high-salt/hypotonic fractionation method for nuclear protein extraction is based on the differential solubility of cellular components. Cytoplasmic proteins are extracted first using a hypotonic buffer that causes cell swelling and membrane rupture, followed by centrifugation to separate the cytoplasmic supernatant from the nuclear pellet. The nuclear pellet is then subjected to high-salt extraction (e. g. , 0. 4 M (NH4)2SO4 or 1 M NaCl) to solubilize tightly bound nuclear matrix proteins, including transcription factors, histones, and structural proteins associated with chromatin and the nuclear scaffold. This approach allows for the isolation of both soluble cytoplasmic proteins and salt-resistant nuclear proteins while minimizing cross-contamination.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Nurr1 agonist 5 Related Classifications
HY-149608 Related Classifications
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