11-Hydroxycanthin-6-one
11-Hydroxycanthin-6-one, a canthin-6-one (HY-N3536) alkaloid, is an antitumor agent which shows potent cytotoxic activity.
For research use only. We do not sell to patients.
- CAS No.: 75969-83-4
- Formula: C14H8N2O2
- Molecular Weight:236.23
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| KB | ED50 |
<=4 μg/mL
Compound: 1
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 3760882] |
| KB | ED50 |
2.1 μg/mL
Compound: 5
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 6619884] |
| P388 | ED50 |
2.3 μg/mL
Compound: 5
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Cytotoxicity against mouse P388 cells
Cytotoxicity against mouse P388 cells
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[PMID: 6619884] |
Chemical Information
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CAS No. 75969-83-4
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Molecular Weight 236.23
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Formula C14H8N2O2
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SMILES
O=C1N2C3=C(N=CC=C3C4=C2C=CC=C4O)C=C1
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
[1]. Fukamiya N, et, al. Antitumor agents: LXXXVII. Cytotoxic antileukemic canthin-6-one alkaloids from Brucea antidysenterica and the structure activity relationships of their related derivatives. Planta Med. 1987 Apr;53(2):140-3. [Content Brief]
[2]. Miyake K, et, al. Canthin-6-one alkaloids and a tirucallanoid from Eurycoma longifolia and their cytotoxic activity against a human HT-1080 fibrosarcoma cell line. Nat Prod Commun. 2010 Jan;5(1):17-22. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
11-Hydroxycanthin-6-one Related Classifications
HY-116786 Related Classifications
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