Cat. No.: HY-N3536

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Canthin-6-one

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Canthin-6-one is an indole alkaloid, displays antibacterial and anti-inflammatory activities.

For research use only. We do not sell to patients.

Purity: 99.92%
CAS No.: 479-43-6
Formula: C₁₄H₈N₂O
Molecular Weight:220.23

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Canthin-6-one Chemical Structure

CAS No.: 479-43-6

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Sample (0.1mg)

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(In Japan)

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Canthin-6-one is an indole alkaloid, displays antibacterial and anti-inflammatory activities^[1]^[2].

All NO Synthase Isoforms :

All COX Isoforms :

All Akt Isoforms :

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	3.6 μg/mL Compound: 5	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 14575431]
ADR5000 cell line	IC₅₀	10.52 μM Compound: 25	Cytotoxicity against human multidrug-resistant CEM/ADR5000 cells assessed as fluorescence incubated for 72 hrs by resazurin reduction assay Cytotoxicity against human multidrug-resistant CEM/ADR5000 cells assessed as fluorescence incubated for 72 hrs by resazurin reduction assay	[PMID: 34332400]
C6	EC₅₀	15 μM Compound: 1	Antiproliferative activity against rat C6 cells assessed as inhibition of cell growth at 10 to 60 uM after 4 days Antiproliferative activity against rat C6 cells assessed as inhibition of cell growth at 10 to 60 uM after 4 days	[PMID: 25379743]
Calu-3	CC₅₀	>100 μM Compound: Table 1, R11C2	Cytotoxicity against human Calu-3 cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay Cytotoxicity against human Calu-3 cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay	[PMID: 36399766]
CCRF-CEM	IC₅₀	15.82 μM Compound: 25	Cytotoxicity against human drug sensitive CCRF-CEM cells assessed as fluorescence incubated for 72 hrs by resazurin reduction assay Cytotoxicity against human drug sensitive CCRF-CEM cells assessed as fluorescence incubated for 72 hrs by resazurin reduction assay	[PMID: 34332400]
DU-145	IC₅₀	9.43 μg/mL Compound: 25	Antiproliferative activity against human DU-145 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human DU-145 cells incubated for 48 hrs by MTT assay	[PMID: 34332400]
HCC1395	IC₅₀	8.12 μg/mL Compound: 25	Antiproliferative activity against human HCC1395 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human HCC1395 cells incubated for 48 hrs by MTT assay	[PMID: 34332400]
HCT-8	CC₅₀	42.41 μM Compound: Table 1, R11C2	Cytotoxicity against human HCT-8 cells assessed as redcution in cell viability incubated for 96 hrs by PrestoBlue reagent based assay Cytotoxicity against human HCT-8 cells assessed as redcution in cell viability incubated for 96 hrs by PrestoBlue reagent based assay	[PMID: 36399766]
HeLa	EC₅₀	15 μM Compound: 1	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth at 10 to 60 uM after 4 days Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth at 10 to 60 uM after 4 days	[PMID: 25379743]
HepG2	CC₅₀	85.65 μM Compound: Table 1, R11C2	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay	[PMID: 36399766]
HT-29	EC₅₀	15 μM Compound: 1	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth at 10 to 60 uM after 4 days Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth at 10 to 60 uM after 4 days	[PMID: 25379743]
Jurkat	EC₅₀	15 μM Compound: 1	Antiproliferative activity against human Jurkat cells assessed as inhibition of cell growth at 10 to 60 uM after 4 days Antiproliferative activity against human Jurkat cells assessed as inhibition of cell growth at 10 to 60 uM after 4 days	[PMID: 25379743]
Keratinocyte	IC₅₀	1.11 μg/mL Compound: 25	Cytotoxicity against guinea pig ear keratinocytes using [3H]thymidine by liquid scintillation counting analysis Cytotoxicity against guinea pig ear keratinocytes using [3H]thymidine by liquid scintillation counting analysis	[PMID: 34332400]
MCF7	ED₅₀	7.3 μg/mL Compound: 5	Cytotoxicity against human MCF7 cells by SRB assay Cytotoxicity against human MCF7 cells by SRB assay	[PMID: 14575431]
MRC5	CC₅₀	91.16 μM Compound: Table 1, R11C2	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay	[PMID: 36399766]
NHDF	EC₅₀	115 μM Compound: 1	Toxicity against human NHDF assessed as reduction in cell viability incubated for 3 days by MTT assay Toxicity against human NHDF assessed as reduction in cell viability incubated for 3 days by MTT assay	[PMID: 25379743]
NIH3T3	EC₅₀	15 μM Compound: 1	Antiproliferative activity against mouse NIH/3T3 cells assessed as inhibition of cell growth at 10 to 60 uM after 4 days Antiproliferative activity against mouse NIH/3T3 cells assessed as inhibition of cell growth at 10 to 60 uM after 4 days	[PMID: 25379743]
PC-3	EC₅₀	15 μM Compound: 1	Antiproliferative activity against human PC3 cells assessed as inhibition of cell growth at 10 to 60 uM after 4 days Antiproliferative activity against human PC3 cells assessed as inhibition of cell growth at 10 to 60 uM after 4 days	[PMID: 25379743]
Vero	CC₅₀	>100 μM Compound: Table 1, R11C2	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay	[PMID: 36399766]

In Vitro

Canthin-6-one exhibits antibacterial activity against Mycobacterium sp. and Staphylococcus sp. with MIC of 8-32 µg/mL^[1].
Canthin-6-one (0.5-5 µM, 25 h) inhibits LPS (HY-D1056)-induced expression of iNOS, COX-2, MCP-1, TNF-α and IL-6, and inhibits the phosphorylation of IκB and Akt in RAW 264.7 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

RAW 264.7
Concentration:

0.5-5 µM
Incubation Time:

25 h
Result:

Inhibited the expression of iNOS, COX-2, p-IκB and p-Akt.

RT-PCR^[2]

Cell Line:

RAW 264.7
Concentration:

0.5-5 µM
Incubation Time:

25 h
Result:

Downregulated the expression of iNOS, COX-2, MCP-1, TNF-α and IL-6.

Chemical Information

CAS No. 479-43-6
Appearance Solid
Molecular Weight 220.23
Formula C₁₄H₈N₂O
Color White to light yellow
SMILES

O=C1N2C3=C(N=CC=C3C4=C2C=CC=C4)C=C1
Structure Classification
- Alkaloids
- Quinoline Alkaloids
- Alkaloids
- Indole Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (113.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.5407 mL

22.7035 mL

45.4071 mL

5 mM

0.9081 mL

4.5407 mL

9.0814 mL

10 mM

0.4541 mL

2.2704 mL

4.5407 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. O'Donnell G, Gibbons S. Antibacterial activity of two canthin-6-one alkaloids from Allium neapolitanum. Phytother Res. 2007;21(7):653-657. [Content Brief]

[2]. Cho SK, et al., Anti-inflammatory Effects of Canthin-6-one Alkaloids from Ailanthus altissima. Planta Med. 2018 May;84(8):527-535. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.5407 mL	22.7035 mL	45.4071 mL	113.5177 mL
	5 mM	0.9081 mL	4.5407 mL	9.0814 mL	22.7035 mL
	10 mM	0.4541 mL	2.2704 mL	4.5407 mL	11.3518 mL
	15 mM	0.3027 mL	1.5136 mL	3.0271 mL	7.5678 mL
	20 mM	0.2270 mL	1.1352 mL	2.2704 mL	5.6759 mL
	25 mM	0.1816 mL	0.9081 mL	1.8163 mL	4.5407 mL
	30 mM	0.1514 mL	0.7568 mL	1.5136 mL	3.7839 mL
	40 mM	0.1135 mL	0.5676 mL	1.1352 mL	2.8379 mL
	50 mM	0.0908 mL	0.4541 mL	0.9081 mL	2.2704 mL
	60 mM	0.0757 mL	0.3784 mL	0.7568 mL	1.8920 mL
	80 mM	0.0568 mL	0.2838 mL	0.5676 mL	1.4190 mL
	100 mM	0.0454 mL	0.2270 mL	0.4541 mL	1.1352 mL

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Canthin-6-one

MCE Bioactive Compound Library

Natural Product Library Plus

High-Throughput Bioactive Compound Library

All NO Synthase Isoforms

All COX Isoforms

All Akt Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Canthin-6-one

MCE Bioactive Compound Library

Natural Product Library Plus

High-Throughput Bioactive Compound Library

All NO Synthase Isoforms

All COX Isoforms

All Akt Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Canthin-6-one Related Classifications

Cat. No.: HY-N3536