Benzyl-α-GalNAc
Based on 13 publication(s) in Google Scholar
Benzyl-α-GalNAc is a potent O-glycosylation inhibitor. Benzyl-α-GalNAc effectively inhibits the proliferation and activation of LX-2 cells and suppresses the expression of collagen I/III, which has good potential for investigation in liver fibrosis. Benzyl-α-GalNAc also significantly enhances the anti-tumour activity of 5-Fluorouracil (HY-90006) (e.g. pancreatic cancer) by inhibiting O-glycosylation.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 3554-93-6
- Formula: C15H21NO6
- Molecular Weight:311.33
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Benzyl-α-GalNAc
More- Cancer Discov. 2026 May 13. [Abstract]
- Acta Pharm Sin B. 2025 Oct 24.
- Adv Sci (Weinh). 2026 Apr;13(19):e16106. [Abstract]
- Adv Sci (Weinh). 2025 Apr;12(15):e2415941. [Abstract]
- Cell Death Differ. 2026 May 25. [Abstract]
- Cell Commun Signal. 2026 Jun 4. [Abstract]
- Cell Mol Gastroenterol Hepatol. 2026 Jan 7;20(5):101721. [Abstract]
- Cell Rep. 2025 Jun 25;44(7):115919. [Abstract]
- Br J Pharmacol. 2026 Aug;183(16):4989-5017. [Abstract]
- Commun Biol. 2025 Apr 17;8(1):626. [Abstract]
- Glycobiology. 2026 May 22;36(7):cwag041. [Abstract]
- Virology. 2024 Apr:592:110008. [Abstract]
- bioRxiv. 2026 Jun 18.
Customer Validation & Images
-
WB
-
IF
-
In Vivo Efficacy Study
-
Histological Imaging/Staining
-
RT-PCR
Biological Activity
Benzyl-α-GalNAc (5 mM; 72 h) inhibits O-glycosylation of mucin in SUIT-2 cells[1].
Benzyl-α-GalNAc (2, 4 mM; 48 h) inhibits the proliferation and activation of LX-2 cells[2].
Benzyl-α-GalNAc (2, 4 mM; 48 h) decreases collagen expression in LX-2 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:SUIT-2 cells
-
Concentration:5 mM
-
Incubation Time:72 h
-
Result:Inhibited mucin O-glycosylation.
-
Cell Line:LX-2 cells
-
Concentration:2, 4 mM
-
Incubation Time:48 h
-
Result:Remarkably decreased the expression of α-SMA in a dose-dependent manner (α-SMA is the marker for the activation of HSCs, which means that quiescent cells transform into myofibroblasts).
-
Cell Line:LX-2 cells (PDGF-BB-induced)
-
Concentration:2, 4 mM
-
Incubation Time:48 h
-
Result:Partly reversed PDGF-BB-induced proliferation in cells.
-
Cell Line:LX-2 cells
-
Concentration:2, 4 mM
-
Incubation Time:48 h
-
Result:Downregulated the mRNA levels of collagens I and III in a dose-dependent manner.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Female severe combined immunodeficient (SCID) mice (6 to 8-week-old; capan-1 tumour model)[3].
-
Dosage:1 mg/mice (in combination with 5-FU)
-
Administration:Tumoural injection; single daily; days 4, 6, 8 and 10 after tumour size reaches 50-70 mm3
-
Result:Significantly lowered neoplastic cells compared to 5-FU alone.
Chemical Information
-
CAS No. 3554-93-6
-
Appearance Solid
-
Molecular Weight 311.33
-
Formula C15H21NO6
-
Color White to off-white
-
SMILES
O[C@@H]([C@H]([C@@H](CO)O1)O)[C@@H](NC(C)=O)[C@H]1OCC2=CC=CC=C2
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (13)
-
Journal Impact Factor
-
Most Recent
-
Cancer Discov
Mismatch Repair-Proficient Colorectal Cancer can evade Immune Surveillance Through an Intrinsic Suppressive Program. [Abstract]2026 May 13. PMID: 42126206 -
-
Adv Sci (Weinh)
GALNT10 Affects O-Glycosylation of IGFBP7 to Promote Tumor Vascular Remodeling and Metastasis of Ovarian Cancer. [Abstract]2026 Apr;13(19):e16106. PMID: 41637621 -
Adv Sci (Weinh)
LIMA1 O-GlcNAcylation Promotes Hepatic Lipid Deposition through Inducing β-catenin-Regulated FASn Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease. [Abstract]2025 Apr;12(15):e2415941. PMID: 39921472
Benzyl-α-GalNAc purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(15):e2415941. [Abstract]
Benzyl-α‐GalNAc (BAGN; 25 mg/kg; i.v.). Western blot analysis of O‐GlcNAc, LIMA1, OGT, OGA, α‐SMA, and Col3A1 in livers from NCD‐fed mice, HFD‐fed mice, and HFD‐fed mice injected with BAGN.
Benzyl-α-GalNAc purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(15):e2415941. [Abstract]
Benzyl-α‐GalNAc (BAGN; 25 mg/kg; i.v.). Immunofluorescence showing LIMA1 (red) and O‐GlcNAc (green) colocalization in livers from NCD‐fed mice, HFD‐fed mice, and HFD‐fed mice injected with BAGN.
Benzyl-α-GalNAc purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(15):e2415941. [Abstract]
Benzyl-α-GalNAc (BAGN; 25 mg/kg; i.v.). Changes in body weight of NCD‐fed mice, HFD‐fed mice, and HFD‐fed mice injected with BAGN.
Benzyl-α-GalNAc purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(15):e2415941. [Abstract]
Benzyl-α-GalNAc (BAGN; 25 mg/kg; i.v.). Tunel and Oil red O staining in livers from NCD‐fed mice, HFD‐fed mice, and HFD‐fed mice injected with BAGN.
Benzyl-α-GalNAc purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(15):e2415941. [Abstract]
Benzyl-α-GalNAc (BAGN; 25 mg/kg; i.v.). Lipometabolic mRNA expression in the liver of NCD‐fed mice, HFD‐fed mice, and HFD‐fed mice injected with BAGN.
-
Cell Death Differ
Targeting ADAR1 lactylation activates innate immune and overcomes chemoresistance in ovarian cancer. [Abstract]2026 May 25. PMID: 42185630 -
Cell Commun Signal
Heterodimerization with signaling-inert TLR8b converts TLR8a from proviral to antiviral sensor in Ctenopharyngodon idella. [Abstract]2026 Jun 4. PMID: 42243884 -
Cell Mol Gastroenterol Hepatol
OGT-enriched Hepatocyte-derived Extracellular Vesicles Promote Capillarization of Liver Sinusoidal Endothelial Cells in Metabolic Dysfunction-associated Steatotic Liver Disease. [Abstract]2026 Jan 7;20(5):101721. PMID: 41513003 -
Cell Rep
Inhibiting UPF1 methylation enhances tumor immunotherapy sensitivity by reducing nonsense-mediated mRNA decay. [Abstract]2025 Jun 25;44(7):115919. PMID: 40570371 -
Br J Pharmacol
Enzyme-responsive 612-AYR nanoparticles for targeted inhibition of B4GALT3-mediated ITGB1 glycosylation in hepatocellular carcinoma. [Abstract]2026 Aug;183(16):4989-5017. PMID: 42157415 -
Commun Biol
The role of ladderlectin in spermatogenesis and ovarian sperm storage in the black rockfish (Sebastes schlegelii). [Abstract]2025 Apr 17;8(1):626. PMID: 40247087 -
Glycobiology
Sialylation profile and Siglec-E expression across tissues in the B16OVA melanoma mouse model. [Abstract]2026 May 22;36(7):cwag041. PMID: 42223497 -
Virology
Proprotein convertase cleavage of Ictalurid herpesvirus 1 spike-like protein ORF46 is modulated by N-glycosylation. [Abstract]2024 Apr:592:110008. PMID: 38335866 -
Solvent & Solubility
DMSO : ≥ 100 mg/mL (321.20 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 7 mg/mL (22.48 mM; ultrasonic and warming and heat to 60°C)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (282 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Kalra AV, et al. Mucin impedes cytotoxic effect of 5-FU against growth of human pancreatic cancer cells: overcoming cellular barriers for therapeutic gain. Br J Cancer. 2007 Oct 8;97(7):910-8. Epub 2007 Oct 2. [Content Brief]
[2]. Kalra AV, et al. Mucin overexpression limits the effectiveness of 5-FU by reducing intracellular drug uptake and antineoplastic drug effects in pancreatic tumours. Eur J Cancer. 2009 Jan;45(1):164-73. [Content Brief]
[3]. Fan X, et al. Protein O glycosylation regulates activation of hepatic stellate cells. Inflammation. 2013 Dec;36(6):1248-52. https://pubmed.ncbi.nlm.nih.gov/23743764/ [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.2120 mL | 16.0601 mL | 32.1203 mL | 80.3006 mL |
| 5 mM | 0.6424 mL | 3.2120 mL | 6.4241 mL | 16.0601 mL | |
| 10 mM | 0.3212 mL | 1.6060 mL | 3.2120 mL | 8.0301 mL | |
| 15 mM | 0.2141 mL | 1.0707 mL | 2.1414 mL | 5.3534 mL | |
| 20 mM | 0.1606 mL | 0.8030 mL | 1.6060 mL | 4.0150 mL | |
| DMSO | 25 mM | 0.1285 mL | 0.6424 mL | 1.2848 mL | 3.2120 mL |
| 30 mM | 0.1071 mL | 0.5353 mL | 1.0707 mL | 2.6767 mL | |
| 40 mM | 0.0803 mL | 0.4015 mL | 0.8030 mL | 2.0075 mL | |
| 50 mM | 0.0642 mL | 0.3212 mL | 0.6424 mL | 1.6060 mL | |
| 60 mM | 0.0535 mL | 0.2677 mL | 0.5353 mL | 1.3383 mL | |
| 80 mM | 0.0402 mL | 0.2008 mL | 0.4015 mL | 1.0038 mL | |
| 100 mM | 0.0321 mL | 0.1606 mL | 0.3212 mL | 0.8030 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Benzyl-α-GalNAc Related Classifications
HY-129389 Related Classifications
Powered by Bioz