OSu-PEG4-VC-PAB-MMAE

Cat. No.: HY-148426: FAQs
Data Sheet SDS Handling Instructions

Molarity Calculator

OSu-PEG4-VC-PAB-MMAE involves in GDP-FAmP₄MMAE (Monomethylauristatin E) synthesis, which is for GDP label via site-specific conjugation.

For research use only. We do not sell to patients.

OSu-PEG4-VC-PAB-MMAE Chemical Structure

CAS No. : 2762518-86-3

Size	Price	Stock
1 mg	USD 350	Ask For Quote & Lead Time
5 mg	USD 950	Ask For Quote & Lead Time
10 mg	USD 1500	Ask For Quote & Lead Time
25 mg	USD 3200	Ask For Quote & Lead Time

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Biological Activity
Purity & Documentation
References
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Description

OSu-PEG4-VC-PAB-MMAE involves in GDP-FAmP₄MMAE (Monomethylauristatin E) synthesis, which is for GDP label via site-specific conjugation^[1].

Molecular Weight

1496.78

Formula

C₇₄H₁₁₇N₁₁O₂₁

CAS No.

2762518-86-3

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H](C1=CC=CC=C1)[C@@H](C)NC([C@H](C)[C@H]([C@@]2([H])N(CCC2)C(C[C@@H](OC)[C@H]([C@@H](C)CC)N(C)C([C@H](C(C)C)NC([C@H](C(C)C)N(C)C(OCC3=CC=C(C=C3)NC([C@H](CCCNC(N)=O)NC([C@H](C(C)C)NC(CCOCCOCCOCCOCCC(ON4C(CCC4=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)OC)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-80°C, protect from light, stored under nitrogen

Solvent & Solubility

In Vitro:

DMSO : 80 mg/mL (53.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1.67 mg/mL (1.12 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.6681 mL	3.3405 mL	6.6810 mL
5 mM	0.1336 mL	0.6681 mL	1.3362 mL
10 mM	0.0668 mL	0.3341 mL	0.6681 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2 mg/mL (1.34 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (1.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2 mg/mL (1.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Data Sheet (275 KB) SDS (251 KB) Handling Instructions (2659 KB)

References

[1]. Yang, et al. Preparation of site-specific antibody conjugates: World Intellectual Property Organization, WO2022037665[P]. 2022-02-24.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.6681 mL	3.3405 mL	6.6810 mL	16.7025 mL
DMSO	5 mM	0.1336 mL	0.6681 mL	1.3362 mL	3.3405 mL
	10 mM	0.0668 mL	0.3341 mL	0.6681 mL	1.6703 mL
	15 mM	0.0445 mL	0.2227 mL	0.4454 mL	1.1135 mL
	20 mM	0.0334 mL	0.1670 mL	0.3341 mL	0.8351 mL
	25 mM	0.0267 mL	0.1336 mL	0.2672 mL	0.6681 mL
	30 mM	0.0223 mL	0.1114 mL	0.2227 mL	0.5568 mL
	40 mM	0.0167 mL	0.0835 mL	0.1670 mL	0.4176 mL
	50 mM	0.0134 mL	0.0668 mL	0.1336 mL	0.3341 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.