p-Nitrobenzyl mesylate
Based on 1 Customer Validation
p-Nitrobenzyl mesylate (4-Nitrobenzyl mesylate) is an alkylating reagent. p-Nitrobenzyl mesylate alkylates thiophosphorylated serine, threonine, tyrosine residues and cysteine thiols, without modifying hydroxyl, amino or carboxylic acid functional groups. p-Nitrobenzyl mesylate generates thiophosphate epitopes on thiophosphorylated substrate proteins for recognition by thiophosphate-specific antibodies. p-Nitrobenzyl mesylate facilitates the identification and purification of kinase substrates. p-Nitrobenzyl mesylate is applicable to studies related to cerebral ischemia-reperfusion injury.
For research use only. We do not sell to patients.
- Purity: 98.78%
- CAS No.: 39628-94-9
- Formula: C8H9NO5S
- Molecular Weight:231.23
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
p-Nitrobenzyl mesylate (2.5 mM; 1 h) achieves complete monoalkylation of a model thiophosphopeptide[1].
p-Nitrobenzyl mesylate alkylates cysteine residues and thiophosphorylated serine residues in c-Jun-GST, but does not react with alcohol, amine, or carboxylic acid functional groups in the protein[1].
p-Nitrobenzyl mesylate (2.5 mM; 2 h) effectively alkylates thiophosphorylated Arabidopsis substrates VQ4 and MKS1, allowing specific detection via anti-TPE antibody to confirm that mutation of MPK gatekeeper residues to alanine does not alter substrate specificity of Arabidopsis MPK3, MPK4, and MPK6[3].
p-Nitrobenzyl mesylate (2.5 mM; 2 h) effectively alkylates thiophosphorylated Arabidopsis substrates AT1G78150, VQ4, and MKS1, allowing specific detection via anti-TPE antibody to confirm differential substrate preferences of Arabidopsis AS-MPK3, AS-MPK4, and AS-MPK6[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 39628-94-9
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Appearance Solid
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Molecular Weight 231.23
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Formula C8H9NO5S
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Color Light yellow to yellow
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SMILES
CS(OCC1=CC=C(C=C1)[N+]([O-])=O)(=O)=O
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Synonyms
4-Nitrobenzyl mesylate; para-Nitrobenzyl mesylate; PNBM
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (432.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Allen JJ, et al. A semisynthetic epitope for kinase substrates. Nat Methods. 2007 Jun;4(6):511-6. [Content Brief]
[2]. Kumar V, et al. Generation and characterization of ATP analog-specific protein kinase Cδ. J Biol Chem. 2015;290(4):1936-1951. [Content Brief]
[3]. Leissing F, et al. Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases. BMC Plant Biol. 2016;16:48. Published 2016 Feb 24. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3247 mL | 21.6235 mL | 43.2470 mL | 108.1175 mL |
| 5 mM | 0.8649 mL | 4.3247 mL | 8.6494 mL | 21.6235 mL | |
| 10 mM | 0.4325 mL | 2.1623 mL | 4.3247 mL | 10.8117 mL | |
| 15 mM | 0.2883 mL | 1.4416 mL | 2.8831 mL | 7.2078 mL | |
| 20 mM | 0.2162 mL | 1.0812 mL | 2.1623 mL | 5.4059 mL | |
| 25 mM | 0.1730 mL | 0.8649 mL | 1.7299 mL | 4.3247 mL | |
| 30 mM | 0.1442 mL | 0.7208 mL | 1.4416 mL | 3.6039 mL | |
| 40 mM | 0.1081 mL | 0.5406 mL | 1.0812 mL | 2.7029 mL | |
| 50 mM | 0.0865 mL | 0.4325 mL | 0.8649 mL | 2.1623 mL | |
| 60 mM | 0.0721 mL | 0.3604 mL | 0.7208 mL | 1.8020 mL | |
| 80 mM | 0.0541 mL | 0.2703 mL | 0.5406 mL | 1.3515 mL | |
| 100 mM | 0.0432 mL | 0.2162 mL | 0.4325 mL | 1.0812 mL |
p-Nitrobenzyl mesylate Related Classifications
HY-118380 Related Classifications
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- p-Nitrobenzyl
- 39628-94-9
- 4-Nitrobenzyl
- para-Nitrobenzyl
- PNBM
- Biochemical Assay Reagents
- thiophosphorylated serine
- cysteine thiols
- mouse bone marrow neutrophil lysates
- Arabidopsis MPK3
- kinase substrates
- tyrosine residues
- cerebral ischemia reperfusion injury
- thiophosphate ester epitopes
- Arabidopsis MPK4
- threonine
- Inhibitor
- inhibitor
- inhibit