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  4. Pimonidazole

Pimonidazole is a novel hypoxia marker for complementary study of tumor hypoxia and cell proliferation in tumor. Pimonidazole accumulates in hypoxic cells via covalent binding with macromolecules or by forming reductive metabolites after reduction of its nitro group, it can be used for qualitative and quantitative assessment of tumor hypoxia .

For research use only. We do not sell to patients.

CAS No. : 70132-50-2

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Pimonidazole:

Pimonidazole Related Antibodies

Top Publications Citing Use of Products

    Pimonidazole purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2025 Aug 28;702(Pt 1):138870.  [Abstract]

    Representative fluorescence images of tumor slices from mice bearing 4 T1 tumors stained with Pimonidazole hydrochloride (PIMO) (60 mg/kg; i.p.; 90 min).

    Pimonidazole purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2025 Dec 3:706:139602.

    The hypoxia level in 4T1 cells was quantitatively assessed using Pimonidazole hydrochloride (PIMO) (100 μM; 60 min) as a probe.

    Pimonidazole purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2025 Dec 3:706:139602.

    Representative fluorescence images of tumor slices from 4 T1 tumor-bearing mice after various treatments were stained for hypoxia with Pimonidazole hydrochloride (PIMO) (60 mg/kg; i.p.; 90 min).

    Pimonidazole purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2024 May:661:908-922.  [Abstract]

    Fluorescence images of tumor slices from mice bearing 4T1 tumors stained with the hypoxia probe Pimonidazole hydrochloride (PIMO) (60 mg/kg; i.p.; 90 min) after different treatments were shown.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Pimonidazole is a novel hypoxia marker for complementary study of tumor hypoxia and cell proliferation in tumor[1]. Pimonidazole accumulates in hypoxic cells via covalent binding with macromolecules or by forming reductive metabolites after reduction of its nitro group, it can be used for qualitative and quantitative assessment of tumor hypoxia [2].

    Cellular Effect
    Cell Line Type Value Description References
    CHO-AA8 IC50
    3.03 mM
    Compound: 2
    Aerobic growth inhibition in Chinese hamster cell line AA8
    Aerobic growth inhibition in Chinese hamster cell line AA8
    		[PMID: 7783125]
    In Vitro

    Pimonidazole, the exogenous hypoxia marker, is a 2-nitroimidazole compound, which forms covalent bonds with cellular macromolecules at oxygen levels below 1.3%.
    Detection: Hypoxic cells were recognized by immunohistochemical detection of pimonidazole using a mouse monoclonal antibody. Cell proliferation was detected with a commercially available monoclonal antibody for proliferating cell nuclear antigen (PCNA). Assessment of hypoxia and cell proliferation was made qualitatively with light microscopy and quantitatively using point counting and image analysis software methods.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Pimonidazole Related Antibodies
    Molecular Weight

    254.29

    Formula

    C11H18N4O3
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    OC(CN1C=CN=C1[N+]([O-])=O)CN2CCCCC2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (196.63 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 1 mg/mL (3.93 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.9325 mL 19.6626 mL 39.3252 mL
    5 mM 0.7865 mL 3.9325 mL 7.8650 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.32%

    SDS (392 KB)

    COA (248 KB) HNMR (260 KB) RP-HPLC (256 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.9325 mL 19.6626 mL 39.3252 mL 98.3130 mL
    DMSO 5 mM 0.7865 mL 3.9325 mL 7.8650 mL 19.6626 mL
    10 mM 0.3933 mL 1.9663 mL 3.9325 mL 9.8313 mL
    15 mM 0.2622 mL 1.3108 mL 2.6217 mL 6.5542 mL
    20 mM 0.1966 mL 0.9831 mL 1.9663 mL 4.9156 mL
    25 mM 0.1573 mL 0.7865 mL 1.5730 mL 3.9325 mL
    30 mM 0.1311 mL 0.6554 mL 1.3108 mL 3.2771 mL
    40 mM 0.0983 mL 0.4916 mL 0.9831 mL 2.4578 mL
    50 mM 0.0787 mL 0.3933 mL 0.7865 mL 1.9663 mL
    60 mM 0.0655 mL 0.3277 mL 0.6554 mL 1.6385 mL
    80 mM 0.0492 mL 0.2458 mL 0.4916 mL 1.2289 mL
    100 mM 0.0393 mL 0.1966 mL 0.3933 mL 0.9831 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Pimonidazole Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Pimonidazole70132-50-2Biochemical Assay ReagentsInhibitorinhibitorinhibit

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