Potassiumalum, for cell culture
Based on 1 Customer Validation
Potassiumalum, for cell culture (Aluminum potassium sulfate dodecahydrate, for cell culture) is an egg white adjuvant. Potassiumalum, for cell culture acts as a mordant to help dyes bind to tissue components, enhancing the staining effect and stability during staining. Potassiumalum, for cell culture can induce allergic reactions in mice. Potassium alum, for cell culture can be used in research for bacterial and tissue staining.
For research use only. We do not sell to patients.
- Purity: 98.01%
- CAS No.: 7784-24-9
- Formula: KAl(SO4)2·12H2O
- Molecular Weight:474.39
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Ammonium chloride, AR, 99.5%
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Potassium iodide ,meet Ch.P
Biological Activity
Potassiumalum (4 h) irreversibly inhibits α-amylase (70% reduction), protease (51% reduction), and lipase (31% reduction) activities in midgut extracts of adult Periplaneta americana, with greater inhibitory effects observed in females than males[1].
Potassiumalum can be used as an adjuvant of ovalbumin to induce anaphylaxis in mice.[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Formalized Vibrio anguillarum bacterin adjuvanted with 2.5% Potassiumalum (100-200 μL; i.p.) causes minimal mortality, a significant reduction in growth, mild to moderate organ damage in rainbow trout[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Salmo gairdneri Richardson (approximately 3 cm length, mean weight 1.5 g)[3]
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Dosage:50; 100 μl (adjuvanted with 2.5% Potassiumalum)
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Administration:i.p.
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Result:Reached 67% cumulative mortality at 48 days post-vaccination for the 50 μl adjuvanted dose.
Reached 74% cumulative mortality at 48 days post-vaccination for the 100 μl adjuvanted dose.
Achieved 231% weight increase over six weeks for the 50 μl adjuvanted dose.
Caused severe reactive peritonitis, inflammatory oedema of visceral peritoneum, macrophage infiltration and adjuvant deposition in serosa of ovary, liver, and pancreas, progressive focal degeneration of pancreatic acini, granulomata formation, fibroblast proliferation with collagen deposition, splenic and renal haemopoietic tissue depletion, occasional subcapsular hepatic necrosis, and heavy sequestration of alum into hepatocytes.
Showed no significant additional protection against Vibrio anguillarum challenge compared to non-adjuvanted vaccine.
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Animal Model:Salmo gairdneri Richardson (20 cm length, mean weight 100 g-156 g)[3]
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Dosage:100; 200 μl (adjuvanted with 2.5% Potassiumalum)
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Administration:i.p.
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Result:Reached 5% cumulative mortality at 48 days post-vaccination for the 100 μl adjuvanted dose.
Reached 6% cumulative mortality at 48 days post-vaccination for the 200 μl adjuvanted dose.
Achieved 53% weight increase over six weeks for the 100 μl adjuvanted dose.
Achieved 26% weight increase over six weeks for the 200 μl adjuvanted dose.
Caused peritoneal oedema, macrophage infiltration, distortion of pancreatic acini, focal hepatic necrosis, severe focal necrosis of renal haemopoietic tissue, sequestration of alum into hepatocytes, pancreatic tissue fibrosis, organizing focal necrosis of liver and kidney, increased macrophage activity in peritoneal tissues, reduction in splenic white pulp, and gonad damage with fibrosed tissue contraction by one month post-injection.
Showed no significant additional protection against Vibrio anguillarum challenge compared to non-adjuvanted vaccine.
Chemical Information
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CAS No. 7784-24-9
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Appearance Solid
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Molecular Weight 474.39
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Formula KAl(SO4)2·12H2O
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Color White to off-white
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SMILES
[KAl(SO4)2 ?12H2O]
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Synonyms
Aluminum potassium sulfate dodecahydrate, for cell culture
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 200 mg/mL (421.59 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Salama EM, et al. A Novel Use for Potassium Alum as Controlling Agent Against Periplaneta americana (Dictyoptera: Blattidae). J Econ Entomol. 2015;108(6):2620-2629. [Content Brief]
[2]. Sevki C, et al. Reduction of house-dust mite allergen concentrations in carpets by aluminium potassium sulfate dodecahydrate (alum). Allergy Asthma Proc. 2006;27(5):350-353. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.1080 mL | 10.5399 mL | 21.0797 mL | 52.6993 mL |
| 5 mM | 0.4216 mL | 2.1080 mL | 4.2159 mL | 10.5399 mL | |
| 10 mM | 0.2108 mL | 1.0540 mL | 2.1080 mL | 5.2699 mL | |
| 15 mM | 0.1405 mL | 0.7027 mL | 1.4053 mL | 3.5133 mL | |
| 20 mM | 0.1054 mL | 0.5270 mL | 1.0540 mL | 2.6350 mL | |
| 25 mM | 0.0843 mL | 0.4216 mL | 0.8432 mL | 2.1080 mL | |
| 30 mM | 0.0703 mL | 0.3513 mL | 0.7027 mL | 1.7566 mL | |
| 40 mM | 0.0527 mL | 0.2635 mL | 0.5270 mL | 1.3175 mL | |
| 50 mM | 0.0422 mL | 0.2108 mL | 0.4216 mL | 1.0540 mL | |
| 60 mM | 0.0351 mL | 0.1757 mL | 0.3513 mL | 0.8783 mL | |
| 80 mM | 0.0263 mL | 0.1317 mL | 0.2635 mL | 0.6587 mL | |
| 100 mM | 0.0211 mL | 0.1054 mL | 0.2108 mL | 0.5270 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Potassiumalum, for cell culture Related Classifications
HY-B2241A Related Classifications
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