The Notch pathway is a contact-dependent signaling pathway that controls cell-fate decisions, differentiation, proliferation, and tissue patterning through interactions between membrane-bound Notch receptors and membrane-bound ligands on neighboring cells. Canonical Notch signaling is activated when ligand engagement triggers proteolytic release of the Notch intracellular domain, which enters the nucleus and regulates transcription together with DNA-binding transcriptional complexes. In the canonical mechanism, ligand-dependent Notch activation leads to release of the intracellular Notch domain, and presenilin-dependent γ-secretase activity is required for production of the active intracellular signaling fragment. The released intracellular domain functions as a nuclear signal that converts Notch receptor activation at the membrane into transcriptional regulation of target programs such as HES/HEY-family genes and other context-dependent downstream targets. The literature links Notch p
Notch Pathway
Notch pathway assay is an experimental method used to analyze and identify the activity of the Notch signaling pathway. The Notch pathway plays a key role in processes such as cell differentiation, proliferation, and apoptosis. In order to detect Notch pathway activity, commonly used methods include real-time fluorescence quantitative PCR (qPCR) to detect Notch pathway-related gene expression, Western blot to analyze the translation level of Notch pathway proteins, and immunohistochemistry or immunofluorescence staining to detect Notch pathway proteins in cells or tissues. expression and positioning.
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